Open Access

Analysis and update of the human solute carrier (SLC) gene superfamily

Human Genomics20093:195

DOI: 10.1186/1479-7364-3-2-195

Received: 2 October 2008

Accepted: 2 October 2008

Published: 1 January 2009

Abstract

The solute-carrier gene (SLC) superfamily encodes membrane-bound transporters. The SLC superfamily comprises 55 gene families having at least 362 putatively functional protein-coding genes. The gene products include passive transporters, symporters and antiporters, located in all cellular and organelle membranes, except, perhaps, the nuclear membrane. Transport substrates include amino acids and oligopeptides, glucose and other sugars, inorganic cations and anions (H+, HCO3-, Cl-, Na+, K+, Ca2+, Mg2+, PO43-, HPO42-, H2PO4-, SO42-, C2O42-, OH-,CO32-), bile salts, carboxylate and other organic anions, acetyl coenzyme A, essential metals, biogenic amines, neurotransmitters, vitamins, fatty acids and lipids, nucleosides, ammonium, choline, thyroid hormone and urea. Contrary to gene nomenclature commonly assigned on the basis of evolutionary divergence http://www.genenames.org/, the SLC gene superfamily has been named based largely on transporter function by proteins having multiple transmembrane domains. Whereas all the transporters exist for endogenous substrates, it is likely that drugs, non-essential metals and many other environmental toxicants are able to 'hitch-hike' on one or another of these transporters, thereby enabling these moieties to enter (or leave) the cell. Understanding and characterising the functions of these transporters is relevant to medicine, genetics, developmental biology, pharmacology and cancer chemotherapy.

Keywords

human genome transporters solute carrier gene superfamily uncoupling proteins mitochondrial proton carriers evolutionary genomics

Introduction

The period between the 1980s and the early 1990s might be considered the era of 'the cloning of genes encoding enzymes and transcription factors', whereas that between the early 1990s and the present day could be regarded as focusing on 'the cloning of genes coding for transporters'. One conceivable reason for the earlier spotlight on many of the enzymes and transcription factors is that those gene products were more abundant and/or could be more easily isolated and antibodies generated against them (compared with transporters). Transporters are embedded within membranes and generally have multiple transmembrane domains. Another reason might be that the mRNA transcripts for enzymes are usually shorter than those for transporters, and early reverse transcription activities starting at the 3' end were tedious and less efficient, meaning that longer mRNA transcripts were often unsuccessful.

Proteins with transport functions http://www.tcdb.org/tcdb/ can roughly be divided into three categories: ATP-powered pumps, ion channels and transporters. ATP-binding cassette (ABC) pumps and other ATP-binding pumps use energy released by ATP hydrolysis to move substrates across membranes and out of cells or into cellular vesicles against their electrochemical gradient. These pumps have two states -- open and closed. By contrast, ion channels in most cases exist in the closed state. Substrates (ions or water) are transferred down their electrochemical gradient at extremely high efficiency (up to 108 s-1). There are 49 ABC-related functional genes in the human genome (including the genes encoding the cystic fibrosis transmembrane conductance regulator [CFTR] and the transporter associated with antigen processing [TAP] 1 and TAP2). Aquaporins (AQPs) are water-channel proteins, encoded by each of 13 AQP functional genes in the human genome http://www.gene-names.org/.

Transporters facilitate the movement of a specific substrate -- either with or against its concentration gradient. It is generally believed that conformational change of the transporter protein is important in this transfer process. Transporters move molecules at only about 102 to 104 s-1, a rate considerably slower than that associated with channel proteins. Many of these transporters belong to the solute-carrier (SLC) gene superfamily -- and include passive transporters, symporters and antiporters, as well as mitochondrial and vesicular transporters. Passive transporters (or uniporters or facilitative transporters) transport one molecule at a time down a concentration gradient. By contrast, active transporters (or co-transporters) couple the movement of one type of ion or molecule against its concentration gradient, to the movement of another ion or molecule down its concentration gradient. (Like ATP pumps, co-transporters mediate coupled reactions in which an energetically unfavourable reaction is coupled to an energetically favourable reaction.) When the transported molecule or ion and the co-transported molecule or ion move in the same direction across a membrane, the transporter is called a symporter; when they move in opposite directions, the transporter is called an antiporter (or exchanger). If the intracellular net charge following transport becomes negative, the process is termed electronegative; if the intracellular net charge becomes positive, the process is called electropositive; if the resulting intracellular net charge remains unchanged, the process is termed electroneutral.

Genes from all these categories are ancient, having members present in most, if not all, prokaryotes, as well as all eukaryotes. Transporters in eukaryotic cells move ions and other molecules across all cellular membranes (cell surface, mitochondrial, endoplasmic reticulum, Golgi and other vesicles), with the possible exception of nuclear membranes (which have pores). The portion of the cell exposed to the lumen is called its apical surface; the rest of the cell (ie its sides and base) make up the basolateral surface. Movement of ions or other molecules into the cell is called influx; movement of ions or other molecules out of the cell is termed efflux.

SLCgene superfamily

Although several specific families within the SLC superfamily have been reviewed during the past year or two, an overview of the entire gene superfamily has not been attempted since Hediger's publication [1] and the special 2004 issue of Pflugers Archives, which was devoted entirely to most of the SLC gene families. Such an update at the present time is deemed important because the number of genes now known to be in the SLC superfamily has changed considerably since then http://www.tcdb.org/hgnc_explore2.php?stem=SLC.

Currently, there are 55 families in the human SLC gene superfamily, with a total of at least 362 putatively functional protein-coding genes. At least 20-25 per cent amino acid sequence identity (most of which occurs in the consensus domain) is shared by member proteins belonging to the same SLC gene family. Table 1 includes the Pfam number http://pfam.sanger.ac.uk/, consensus sequence (or domain) and GenBank accession number for the first member of the 55 genes/gene products. Note that the SLC35, SLCO1, SLCO2 and SLCO4 families contain two or more subfamilies, whereas the remaining 51 families have no subfamilies (Table 1). In most families where more than one member is present, the first member was chosen to represent that entire family for the global amino acid alignment of the 55 proteins to generate a nearest-neighbour-joining (NNJ) dendrogram (Figure 1).
Table 1

Human SLC gene superfamily, including description of the gene products

Protein

family

Description

Number of

members

Pfam

Domain

GenBank

accession

number

SLC1

High-affinity glutamate and neutral amino acid transporter family

7

PF00375

SDF

NP_004161

SLC2

Facilitative glucose transporter (GLUT) family

14; 5 pseudo

PF00083

Sugar_tr

NP_006507

SLC3

Heavy subunits of the heteromeric amino acid transporters

2

PF00128

Alpha-amylase

NP_000332

SLC4

Bicarbonate transporter family

10

PF07565

Band_3_cyto

NP_000333

   

PF00955

HC03_cotransp

 

SLC5

Na+/glucose co-transporter family

12

PF00474

SSF

NP_000334

SLC6

Na+- and Cl--dependent neurotransmitter symporter family

19; 3 pseudo

PF00209

SNF

NP_003033

SLC7

Cationic amino acid transporter/glycoprotein- associated amino acid transporter family

14; 1 pseudo

PF00324

AA_permease

NP_003036

SLC8

Na+/Ca2+ exchanger family

3

PF03160

Na_Ca_ex

NP_066920

   

PF01699

Calx_eta

 

SLC9

Na+/H+ exchanger family

11; 4 pseudo

PF00999

Na_H_Exchanger

 

SLC10

Na+/bile salt co-transporter family

7

PF01758

SBF

NP_003040

SLC11

Proton-coupled metal ion transporter family

2

PF01566

Nramp

NP_000569

SLC12

Electroneutral cation/Cl- co-transporter family

9

PF00324

AA_permease

NP_000329

   

PF08403

  

SLC13

Na+/SO42-/carboxylate co-transporter family

5

PF00939

Na_sulph_symp

NP_071889

SLC14

Urea transporter family

2

PF03253

UT

NP_056949

SLC15

Proton oligopeptide co-transporter family

4

PF00854

PTR2

NP_005064

SLC16

Monocarboxylate transporter family

14

PF07690

MFS_1

NP_003042

SLC17

Vesicular glutamate transporter family

8

PF07690

MFS_1

NP_005065

SLC18

Vesicular amine transporter family

3

PF07690

MFS_1

NP_003044

SLC19

Folate/thiamine transporter family

3

PF01770

Folate_carrier

NP_919231

SLC20

Type-III Na+/H2PO4- co-transporter family

2; 1 pseudo

PF01384

PH04

NP_006740

SLC22

Organic cation/anion/zwitterion transporter family

23

PF07690

MFS_1

NP_003048

SLC23

Na+-dependent ascorbic acid transporter family

4

PF00860

Xan_ur_permease

NP_005838

SLC24

Na+/Ca2+ and Na+/K+ exchanger family

6

PF01699

Na_Ca_ex

NP_004718

SLC25

Mitochondrial carrier family

43; 4 pseudo

PF00153

Mito_carr

NP_005975

SLC26

Multifunctional anion exchanger family

11

PF01740

Sulfate_transp

NP_998778

   

PF00916

STAS

 

SLC27

Fatty acid transport protein family

6

PF00501

AMP-binding

NP_940982

SLC28

Na+-coupled nucleoside transport family

3

PF07670

Gate

NP_004204

   

PF07662

Nucleos_tra2_C

 
   

PF01773

  

SLC29

Facilitative nucleoside transporter family

4

PF01733

Nucleoside_tran

NP_001071642

SLC30

Zn2+ efflux family

10

PF01545

Cation_efflux

NP_067017

SLC31

Copper transporter family

2; 1 pseudo

PF04145

Ctr

NP_001850

SLC32

Vesicular inhibitory amino acid transporter family

1

PF01490

Aa_trans

NP_542119

SLC33

Acetyl coenzyme A transporter family 22

1

IPR004752

2A0125

NP_004724

SLC34

Type-II Na+/HPO42- co-transporter family

3

PF02690

Na_Pi_cotrans

NP_003043

SLC35

(A, B, C, D, E, F)

Nucleoside-sugar transporter family

23

PF04142

Nuc_sug_transp

NP_006407

SLC36

Proton-coupled amino acid transporter family

4

PF01490

Aa_trans

NP_510968

SLC37

Sugar-PO43-/PO43- exchanger family

4

PF07690

MFS_1

NP_061837

SLC38

System A & N, Na+-coupled neutral amino acid transporter family

11

PF01490

Aa_trans

NP_109599

SLC39

Metal (M2+) ion transporter family

14

PF02535

Zip

NP_055252

SLC40

Basolateral iron transporter family

1

PF06963

FPN1

NP_055400

SLC41

MgtE-like Mg2+ transporter family

3

PF01769

MgtE

NP_776253

SLC42

Rh-associated glycoproteins; NH4+ transporter family

3

   

SLC43

Na+-independent system-L-like amino acid transporter family

3

  

NP_003618

SLC44

Choline-like transmembrane transporter activity

5

PF04515

DUF580

NP_536856

SLC45

Putative sugar/H+ symporter activity

4

PF07690

MFS_1

NP_001073866

SLC46

Folic acid transporter (heme-containing) activity

3

PF07690

MFS_1

NP_542400

SLC47

Polyspecific H+/organic cation exporter

2

PF01554

MatE

NP_060712

SLCO1

(A, B, C)

Drug, organic anion transporter family

4

PF07648

OATP

NP_602307

   

PF03137

  

SLCO2

Prostaglandin and steroid sulphate transporter family

2

PF07648

OATP

NP_005621

   

PF03137

  

SLCO3

Drug and organic anion transporter activity

1

PF07648

OATP

NP_037404

   

PF03137

KAzal_2

 

SLCO4

Thyroid hormone transmembrane transporter activity

2

PF07648

OATP

NP_057438

   

PF03137

  

SLCO5

Drug and organic anion transporter activity

1

PF07648

OATP

NP_112220

   

PF03137

  

SLCO6

Drug and organic anion transporter activity

1

PF07648

OATP

NP_775759

   

PF03137

Kazal_2

 

UCP1

Oxidative phosphorylation uncoupling activity (proton carrier)

1

PF00153

Mito_carr

NP_068605

UCP2

Oxidative phosphorylation uncoupling activity (proton carrier)

1

PF00153

Mito_carr

NP_003346

UCP3

Oxidative phosphorylation uncoupling activity (proton carrier)

1

PF00153

Mito_carr

NP_003347

The numbers of documented pseudogenes ('pseudo'), to date, are noted in the 'Number of members' column; these numbers are likely to be gross underestimates, however, because if one does a BLAST search with each exon, numerous 'bits and pieces' of the gene plus detritus exons will probably be discovered [2].

Figure 1

Dendrogram of a representative member of each of the 55 human SLC gene families, developed using Clustal W software, to test for evolutionary readiness. To avoid clutter, we have selected only the first member of each family, although most families have two or more members. We also added two unrelated 'outlier' genes (SOD1 and CYP1A1) and two additional members of the SLC39 family (SLC39A2 and SLC39A3) as 'internal controls'. This nearest neighbour-joining (NNJ) method uses only global alignments of the entire protein sequences. In this case, although the NNJ method appears to gives various branches of different lengths, reflecting the presumed time since evolutionary divergence of the various branches of the gene tree, this tree is largely an artefact because the superfamily has mainly been pulled together by nomenclature experts who based this superfamily on function, rather than evolutionary divergence (see text).

What had originally been named the 'SLC21 (organic-anion transporting) family' has now been changed to six highly divergent SLCO families. Also, the SLC42 family has its genes named RHAG, RHBG and RHCG, because they were first characterised as members of the blood Rh factor antigen family (Table 1).

Evolution of the SLCgenes

We examined the SLC superfamily by the NNJ method (Figure 1); we included two functionally unrelated 'outlier' genes (SOD1, encoding a soluble protein, and CYP1A1, encoding a membrane-bound protein) and two 'internal controls' within the same subfamily (SLC39A2 and SLC39A3, together with SLC39A1). SOD1 and CYP1A1 appeared to be 'evolutionarily related' to SLC7 and SLC3, respectively (Figure 1). Thus, whereas the three SLC39 family members are clustered, the unrelated 'outliers' did not fall outside the superfamily tree (as they should). From these findings, we conclude that -- although the NNJ method of analysis suggests an evolutionary tree --one cannot detect sufficient evolutionary relatedness for the vast majority of the 55 SLC families making up this superfamily.

There are two clusters of gene families, however, that do show evolutionary relatedness (Figure 1). One is the mitochondrial-transport SLC25 family (of 43 members) clustered together with the three UCP families. This cluster is undoubtedly real because all members are concerned with transport across mitochondrial membranes. The other cluster is the group of six SLCO families. This is intriguing, especially because (vide infra) the substrates are fairly diverse (organic anions and drugs, prostaglandins, lipids and thyroid hormone).

Beyond those two clusters, we see no other statistically significant evolutionary relatedness. Therefore, with the exception of these two clusters, the remainder of the SLC gene families will be discussed on the basis of their common substrates. Many genes in the SLC superfamily are involved in paediatric inherited disorders and other human diseases (see Bergeron et al. [3] and http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim). In addition, the functional properties of each family are often summarised on the basis of just a few members that have been thoroughly characterised. If that family has, say, 12 or 23 members, we should keep in mind that it is possible that some of the other members yet to be characterised will not adhere strictly to that specific moniker.

Inorganic cation/anion transport

Teleologically, one might argue that inorganic cation and anion transport would be, in evolutionary terms, among the oldest transport functions. Eight families comprise the group that transports exclusively inorganic cations and anions across membranes (Table 1): SLC4, with ten members, plays a pivotal role in mediating Na+- and/or Cl--dependent transport of basic anions (eg HCO3-, CO32-) in various tissues and cell types (in addition to pH regulation, specific members of this family also contribute to vectorial transepithelial base transport in several organ systems, including the kidney, pancreas and eye); [4] SLC8, with three members, is a group of Na+/Ca2+ exchangers (SLC8A1 is known to exchange three extracellular Na+ ions for one intracellular Ca2+ ion and to be involved in cardiac contractility); [5] SLC9, with 11 members, comprises Na+/H+ exchanger proteins involved in the electroneutral exchange of Na+ and H+; [6] SLC12, with nine members, functions as a Na+, K+ and Cl- ion electroneutral symporter; [7, 8] SLC34, with three members, is an important type II Na+/(HPO4)2- symporter; [9, 10] SLC20, with two members, originally identified as a viral receptor, [11] functions as a type III Na+/(H2PO4)- symporter; [10, 11] SLC24, with six members, is a group of Na+/Ca2+ or Na+/K+ exchangers; [12] and SLC26, with 11 members, is the transepithelial multifunctional anion (eg sulfate, oxalate, HCO3-, Cl-) exchanger family, [13, 14] also important in sound amplification in the cochlea [15].

Amino acid and oligopeptide transport

Eight families are involved as transporters of amino acids and/or oligopeptides (Table 1): SLC1, with seven members, transports high-affinity glutamate and neutral amino acids; [3, 16] SLC3, with two members, encodes transporters of heavy subunits of heteromeric amino acids [3, 17]. The SLC3 family (along with other amino acid carrier SLC1, SLC6 and SCL7 families, plus the glucose carrier SLC2 and SLC5 families) is regarded as a collection of transporters that function mainly in 'epithelial-derived' cells; [3] SLC7, with 14 members, represents cationic amino acid/glycoprotein transporters; [3, 18] SLC15, with four members, represents a family of proton-oligopeptide symporters; [19, 20] SLC17, with eight members, is involved in diverse processes ranging from the vesicular storage of the neurotransmitter glutamate to the degradation and metabolism of glycoproteins; [21] SLC32, with one member only, transports amino acids across vesicle membranes; [22] SLC36, with four members (a mutation in the SLC36A1 gene was recently found to be associated with champagne dilution coat colour in horses [23]), is involved in proton-coupled amino acid transport; [24] SLC38, with 11 members, functions as a sodium-coupled neutral amino-acid transporter; [25] and SLC43, with three members, represents the sodium-independent system-L-like (ie mediating the movement of bulky neutral amino acids across cell membranes) amino acid transporter family [1, 26]. It is worth noting that the SLC32, SLC36 and SLC38 families do appear to be evolutionarily related (Figure 1). SLC16 and SLC22 also transport amino acids, among other substrates, and are described later.

Transport of glucose and other sugars

Four families function as sugar transporters (Table 1): SLC2, with 14 members, is the well-studied facilitative glucose transporter (GLUT) family; [2, 27] SLC5, with 12 members, functions as a Na+/glucose symporter; [2, 28] SLC37, with four members, is a group of sugar-PO4-/PO4- exchangers, with glucose-6-PO4- transporter-1 being the most well characterised; [29] and SLC45, with four members, appears to function as a sugar/H+ symporter. The SLC45A1 gene is located at 1p36.23 [30]. SLC45A2, associated with skin pigmentation and protection against malignant melanoma, [31] is located at 5p13.3. SLC45A3, located at 1q32.1, is, curiously, one of several genes that have been found to be involved in recurrent gene rearrangements in prostate cancer [32]. SLC45A4 33 was mapped to 8q24.3 http://www.genenames.org/.

Transport of bile salts and organic anions

Four families participate as transporters of bile salts and organic anions (Table 1): SLC10, with seven members, is involved in bile acid transport; [34] SLC13, with five members, is the Na+/sulphate/selenate/thiosulphate/carboxylate symporter family. The di- and tri-carboxylates include succinate, citrate and alpha-ketoglutarate; [35] SLC16, with 14 members, is involved in the proton-linked transport of monocarboxylate anions (eg lactate, pyruvate and ketone bodies) and aromatic amino acids; [36] and SLC47, with two members, has so far only been characterised as a polyspecific H+/organic cation exporter [37]. The SLC47 genes have also been nicknamed 'multidrug and toxicant extrusion-1 and -2' (MATE1 and MATE2). The SLC47A1 and SLC47A2 genes both map to 17p11.2 (http://www.genenames.org/. Four of the SLCO families also participate in organic anion transport, and these are separately described later as an evolutionary cluster.

Metal ion transport

Six SLC families are involved in metal ion transport (Table 1): SLC11, with two members that function as proton-coupled metal ion influx transporters, also known as the 'natural resistance-associated macrophage protein' (NRAMP) homologues; [38] SLC30, with ten members, is involved in Zn2+ efflux; [39, 40] SLC31, with two members, is a copper influx transporter family; [41] SLC39, with 14 members, functions in the influx of essential metals such as Zn2+, Fe2+, Cu2+ and Mn2+, [40, 42] although non-essential toxic metals such as Cd2+, Pb2+ and Hg2+ can 'hijack' at least two of these transporters; [43, 44] SLC40, with one member only, is a basolateral iron transporter; [45] and SLC41, with three members, is the 'MgtE-like' magnesium transporter family, which has been characterised principally in prokaryotes (also found in yeast, worm and fly), while their physiological role in eukaryotes remains unclear [46].

Transport of urea, neurotransmitters and biogenic amines, ammonium and choline

Five families participate in the transport of these molecules (Table 1): SLC6, with 19 members, represents Na+ and Cl- ion-dependent neurotransmitter (gamma-aminobutyric acid [GABA], serotonin, dopamine and norepinephrine) transporters, [3, 47] having relatives even in prokaryotes; [48] SLC14, with two members, is involved in the transport of urea; [49] SLC18, with three members, transports acetylcholine (by the vesicular acetylcholine transporter SLC18A3) and biogenic amines (by the vesicular monoamine transporters SLC18A1 and SLC18A2) into secretory vesicles, which are then discharged into the extracellular space by exocytosis; [50] SLC22, with 23 members, is highly conserved in the fly and worm, functions in endogenous organic cation/anion/zwitterion (eg carnitine, betaines, amino acids) transport and thus is very important in drug transporter functions; [51] SLC42, with three members [52] that appear to be involved in NH4+ transport (whereas their gene names are RHAG, RHBG and RHCG); and SLC44, with five members and homologues in yeast, fly and worm, appears to be involved in choline transport [53].

Transport of vitamins and cofactors

Four families participate in vitamin or cofactor transport (Table 1): SLC19, with three members, transports folate and thiamine, energised by a trans-membrane H+/OH- gradient; [54] SLC23, with four members, transports ascorbic acid; [55] SLC33, with a single member, is an acetyl coenzyme A transporter, which serves as a substrate of acetyltransferases that modify the sialyl residues of gangliosides and glycoproteins; [56] SLC46, with three members, is involved in proton-coupled folic acid transport. Homozygous mutations in the SLC46A1 gene, located at 17q11.2, are associated with hereditary folate malabsorption [57]. SLC46A2[58] maps to 9q32, and SLC46A3[33, 59] to 13q12.3 http://www.gene-names.org/.

Nucleoside/nucleotide transport

Three families carry out the transport of nucleosides and nucleotides (Table 1): SLC28, with three members, functions in Na+-coupled nucleoside transport and thus is a potentially important pharmacological target; [60] SLC29, with four members, mediates (along with the SLC28 transporters) uptake of natural nucleosides (among them adenosine) -- these members are major routes of entry for a variety of nucleoside analogues used in anticancer and antiviral therapies; [61] SLC35, with 23 members, transports nucleotide sugars (pooled in the cytosol) into the lumen of the Golgi apparatus and endoplasmic reticulum, wherein occurs most of the synthesis of glycoconjugates [62].

Transport of fatty acids, prostaglandins and steroid sulphates

Two SLC families are involved in these functions (Table 1): SLC27, with six members, participates in the transport of long-chain fatty acids; [63] and SLCO2, with two members (detailed below), functions in prostaglandin and steroid sulphate transport.

SLCOgene families

The six SLCO gene families represent an evolutionary cluster (Figure 1); four of the families are involved in organic anion-transporting polypeptides (OATPs), which include 14 transmembrane-domain glycoproteins expressed [64] in various epithelial cells (Table 1): SLCO1, with four members, is involved in drug transport; SLCO3, with a single member, transports unknown organic anions; SLCO5 and SLCO6, both families having a single member, also transport unknown organic anions and are believed to be important in drug transport, whereas SLCO2, with two members, functions in the transport of prostaglandins [65] and steroid sulphates [66]. SLCO4, with two members, functions in the transport of thyroid hormone [67]. Most of the SLCO proteins have not yet been well characterised.

Transport across mitochondrial membranes

The four gene families involved in mitochondrial transport also represent an evolutionary cluster (Figure 1): SLC25, with 43 members (the largest of all SLC families), is known to comprise the 'mitochon-drial carriers', shuttling a variety of metabolites across the mitochondrial inner membrane; [68] and UCP1, [69] UCP2 [70, 71] and UCP3 [70] (located at chromosomes 4q28-q31, 11q13 and 11q13.4, respectively) function as ancient uncoupling proteins, or proton pumps involved in mitochondrial energetics (Table 1).

Conclusions

The SLC gene superfamily comprises 55 families, totalling at last 362 putatively functional protein-coding genes that encode multiple transmembrane transporters. Whereas all the transporters undoubtedly have endogenous substrates, drugs, non-essential metals and many other environmental toxicants in all likelihood are able to 'hitch-hike' on one or another of these transporters, thereby being able to enter (or leave) the cell. Understanding and characterising the functions of all these transporters should be relevant to medicine, genetics, pharmacology and cancer chemotherapy. Because more than half of these genes remain to be characterised, this field seems ripe -- perhaps especially for young investigators who wish to choose a research topic with little or no competition at the present time.

Declarations

Acknowledgements

We thank our colleagues, especially David R. Nelson, for valuable discussions and a critical reading of this manuscript. The writing of this review paper was funded, in part, by NIH grant P30 ES06096 (D.W.N.).

NOTE ADDED IN PROOF

The SLC22A12 gene is known to encode urate transporter-1. The very recent finding of an association of mutations in the SLC2A9 gene from dogs exhibiting hyper uricosuria and hyperuricaemia* underscores the importance of SLC289 as an additional uric acid transporter in mammals, which in all likelihood include humans.

*Bannasch, D., Safra, N., Young, A., Karmi, N., Schaible, R.S. and Ling, G.V. (2008), 'Mutations in the SLC289 gene cause hyperuricosuria and hyperuricemia in the dog', PLoS Genet. 30 November; e1000246.

Authors’ Affiliations

(1)
Massachusetts General Hospital Cancer Center and Harvard Medical School
(2)
Molecular Toxicology and Environmental Health Sciences Program, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center
(3)
Department of Environmental Health and Center for Environmental Genetics (CEG), University of Cincinnati Medical Center

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