Update on the Kelch-like (KLHL) gene family
© Dhanoa et al.; licensee BioMed Central Ltd. 2013
Received: 4 February 2013
Accepted: 30 April 2013
Published: 15 May 2013
The Kelch-like (KLHL) gene family encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain, and five to six Kelch motifs. BTB domains facilitate protein binding and dimerization. The BACK domain has no known function yet is of functional importance since mutations in this domain are associated with disease. Kelch domains form a tertiary structure of β-propellers that have a role in extracellular functions, morphology, and binding to other proteins. Presently, 42 KLHL genes have been classified by the HUGO Gene Nomenclature Committee (HGNC), and they are found across multiple human chromosomes. The KLHL family is conserved throughout evolution. Phylogenetic analysis of KLHL family members suggests that it can be subdivided into three subgroups with KLHL11 as the oldest member and KLHL9 as the youngest. Several KLHL proteins bind to the E3 ligase cullin 3 and are known to be involved in ubiquitination. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. Further investigation of this family of proteins will likely provide valuable insights into basic biology and human disease.
KeywordsKLHL Kelch domain BTB domain Ubiquitination Gene family Evolution Mendelian disease Gene nomenclature Cancer
The KLHL (Kelch-like) gene family encodes proteins that constitute a subgroup at the intersection between the BTB/POZ domain and Kelch domain superfamilies. First identified in the vaccinia virus , the BTB motif was named based on a homologous domain of 115 amino acids in Drosophila melanogaster bric à brac 1, tramtrack, and broad-complex proteins (BTB) . BTB domain-containing zinc finger proteins are conserved evolutionarily from Drosophila to humans and mice with more than 49 family members in the latter two . The POZ domain was initially named after the 120 amino acid motifs present at the amino terminus in poxvirus proteins and zinc finger proteins . The BTB/POZ domain facilitates protein binding  as reviewed in Perez-Torrado et al. . Functions observed to be associated with other BTB-containing proteins involve a variety of cellular mechanisms such as control of cytoskeletal organization , ion channel gating , transcription suppression , and protein targeting for ubiquitination through cullin E3 ligases [10, 11].
The Kelch repeat or domain is also an evolutionarily conserved structure that can be found from Drosophila melanogaster to Homo sapiens. Based on sequence identity, it has been suggested that each four-stranded β-sheet of the Kelch motif forms one blade of a β-propeller structure . The Kelch superfamily of proteins can be subdivided into five groups. These subgroups include (1) N-propeller, C-dimer proteins, (2) N-propeller proteins, (3) propeller proteins, (4) N-dimer, C-propeller proteins, and (5) C-propeller proteins . Kelch-containing proteins have roles in extracellular communication/interaction, cell morphology, gene expression, actin binding, and can be co-opted by virus post-infection . KLHL family members belong to the N-dimer, C-propeller subclass of Kelch repeat proteins.
In addition to BTB/POZ and Kelch domains, the KLHL family members contain a BACK domain, first described as a 130-residue region of conservation observed amongst BTB-Kelch proteins . Although no function has been assigned to the BACK domain, it is likely to be of functional significance because mutations in this region have been shown to cause human disease [14–19].
The BTB superfamily includes KLHL, KBTBD, and KLHDC subfamilies, which encompass structurally related molecules that differ in the types and numbers of their protein domains. In general, KLHL proteins contain one BTB/POZ domain, one BACK domain, and five to six Kelch motifs (BTB, BACK, 5/6 Kelch). KBTBD proteins have one BTB/POZ domain, occasionally a BACK domain, and two to four Kelch motifs (BTB/POZ, (BACK), 2/4 Kelch). Most KLHDC family members contain only three to seven Kelch motifs and usually do not have BTB or BACK domains (3/7 Kelch). Herein, we describe the evolutionary structure of the human KLHL family, discuss examples of KLHL family member biology, and present an overview of KLHL protein relationship to human disease.
Evolutionary structure of the human KLHL family
There are nine KBTBD genes in the human genome, and their encoded proteins typically possess a BTB and BACK domain and two to four Kelch motifs. Ten KLHDC genes have been defined by the HUGO Gene Nomenclature Committee (HGNC) in the human genome. KLHDC proteins generally do not have BTB or BACK domains and have three to seven Kelch motifs. These subfamilies will not be discussed further in this review.
Examples of KLHL family members
The KEAP1 gene is clearly a member of the KLHL family. While the well-used KEAP1 symbol has been retained as the official gene nomenclature; it has also been assigned the KLHL19 synonym to clearly group it within the KLHL family. The human KEAP1 (Kelch-like ECH-associated protein 1 gene is located on chromosome 19p13, and the mouse ortholog is located on chromosome 9. KEAP1 was originally identified as a nuclear factor erythroid 2-related factor 2 (NRF2) interacting protein. NRF2 is a transcription factor that is essential for the cellular reaction to electrophiles and binds to the antioxidant response element (ARE) present in promoters of genes involved in phase II detoxifying and oxidative stress enzyme response . KEAP1 was shown to negatively control NRF2 transactivation potential . Some of the genes controlled by NRF2 are important for cellular defense against harmful oxidative stresses that could result in carcinogenesis (as reviewed in Motohashi and Yamamoto ). NRF2 interacts as a heterodimer with Maf transcription factor protein that binds to the ARE and activates transcription through the Maf recognition element (MARE) (reviewed in Motohashi and Yamamoto ).
KEAP1's ability to repress NRF2's downstream target activation was demonstrated in zebrafish by gain-of-function and loss-of-function experiments in which transcriptional control by NRF2 of glutathione S-transferase gene (gstp) induction was tested in the absence and presence of KEAP1. NRF2-induced gstp expression was repressed when KEAP1 was also overexpressed . KEAP1 was found to promote NRF2 degradation by targeting it for ubiquitination through the cullin 3 ligase complex [28, 29], thus preventing NRF2 activity under normal cellular conditions . Domain function analysis of KEAP1 revealed that NRF2 degradation requires both BTB and intervening domains .
KEAP1 is localized primarily in the cytoplasm of cells, with a minimal amount in the nucleus and endoplasmic reticulum . KEAP1 scaffolding to the actin skeleton is crucial for effective sequestering of NRF2 into the cytoplasm . Electrophiles promote the nuclear accumulation of NRF2 without altering the subcellular localization of KEAP1 . Furthermore, KEAP1 appears to regulate both translational and post-translational responses to oxidative stress [32, 33].
The crystal structure of mouse KEAP1 was resolved to reveal a six-bladed propeller tertiary structure encompassing the Kelch domain . The analysis showed which bonds are needed for structural integrity and proper association between KEAP1 and NRF2 . NRF2 has been suggested to be involved in susceptibility of lungs to cigarette smoke through the induction of antioxidant genes . KEAP1's relationship with cancer is described below.
KLHL2/Mayven is an actin-binding protein that shares 63% identity (77% similarity) with the Drosophila ring canal (Kelch) protein . The human gene is localized on chromosome 4q21.1, and its murine ortholog is on chromosome 8 . KLHL2 RNA can be detected in developing and adult brain, heart, spleen, lung and liver, and adult kidney but most predominantly throughout the brain including the hippocampus, caudate nucleus, corpus callosum, and amygdala . The KLHL2 protein has been shown to be constitutively expressed in developing and mature oligodendrocytes and neurons where it binds directly to F-actin through its Kelch repeats and plays an important role in the organization of the actin cytoskeleton [36, 37]. In developing oligodendrocytes, KLHL2 is upregulated during differentiation , and it localizes to lamellipodia (but not filipodia) . KLHL2 overexpression in oligodendrocyte progenitors accelerates neurite outgrowth and leads to longer processes. Conversely, downregulation inhibits process extension [37, 38]. KLHL2 co-localizes and associates with FYN oncogene related to SRC, FGR, and YES (FYN) tyrosine kinase through its SH3-binding domain at the N-terminus. This interaction increases upon differentiation, suggesting a role in promoting oligodendrocyte process outgrowth and strengthening of the initial axonal-glial contact mediated by FYN signaling. Based on the type of interaction, it has been suggested that KLHL2 acts as a linker between FYN and actin . Upon depolarization of primary hippocampal neurons with KCl, association of KLHL with actin is enhanced, resulting in re-distribution and translocation along the axonal processes . The functional significance of these findings needs further investigation.
Recently, independent interactions with two different proteins have underscored KLHL2 involvement in ubiquitination. KLHL2 binds to nucleus accumbens-associated 1 (NACC1), a transcriptional repressor protein that interacts also with another KLHL family member, ectodermal-neural cortex 1 (ENC1/KLHL37). KLHL2 may form part of the ubiquitin complex through which NACC1 affects degradation of specific gene products and promotes proteasome activity and trafficking . Finally, interactions of KLHL2 have been shown with neuronal pentraxin with chromo domain (NPCD, encoded by the Npcd gene in mouse which is a read-through between Cbx6 and Nptxr) and with cullin 3. In this case, KLHL2 appears to function as a specific adapter for NPCD ubquitination via cullin 3. Notably, overexpression of NPCD in hippocampal neurons leads to apoptosis .
KLHL family members and inherited disease
KLHL members associated with inherited forms of human disease include KLHL3, KLHL7, KLHL9, KLHL12, and GAN (KLHL16). Mutations in KLHL3 have been identified in patients with familial hyperkalemic hypertension. Molecular analyses have shown that most mutations increase the activity of the Na+Cl− symporter in the distal convoluted tubule portion of the nephron. The increased activity causes more Na+ and Cl- reabsorption, increasing blood pressure and culminating in hypertension. Although mutations were identified in this familial disease, screening for single nucleotide polymorphisms (SNPs) in KLHL3 did not reveal any significant association with blood pressure measurements in human subjects, indicating that common variants are not responsible for variations in blood pressure .
Patients with autosomal dominant retinitis pigmentosa (adRP), a neurodegenerative disease that leads to loss of rod and cone photoreceptors, carry mutations in KLHL7. Early and often rapid alteration of rod function is a characteristic of the classic form of adRP, but the phenotype observed in the KLHL7 patients differs because of its late onset and preserved rod function in older family members [42, 43]. Recent work suggests that KLHL7 protein binds to cullin 3 and that a single mutation in the BACK domain leads to reduced efficiency of cullin 3's ubiquitin ligase activity . KLHL7 and KLHL12 have also been identified as autoantigens in Sjögren's syndrome, an autoimmune disease that causes damage to the salivary and lachrymal glands . No significant immunological response to either KLHL7 or KLHL12 was observed in the sera of healthy individuals .
A missense mutation in KLHL9 leads to the development of distal myopathy in human patients. Distal myopathy is a degenerative disease that initially causes atrophy of distal limb muscles and subsequently extends to proximal limbs . Mutations in GAN (KLHL16), or gigaxonin, are linked to human giant axonal neuropathy, an autosomal recessive disorder . Giant axonal neuropathy affects the central and peripheral nervous system that becomes populated with large, dysfunctional axons . Compound heterozygous missense mutations in the Kelch-coding region of gigaxonin have recently been reported in Chinese cases .
KLHL family members and cancer
Four KLHL family members are associated with cancer: KLHL6, KEAP1 (KLHL19), KLHL20, and ENC1 (KLHL37). Mutations in KLHL6 are recurrent in cases of chronic lymphocytic leukemia (CLL), a type of leukemia that is predominant in adults. Six mutations were identified within KLHL6 in CLL patients, and all disrupt KLHL6 function in germinal center B cell formation [48, 49]. Insertion, missense, frameshift, and missense mutations in KEAP1[17, 35] have been identified in cancerous cells of the liver, gallbladder, and lung. Mutations that result in KEAP1 loss of function are thought to facilitate cancer cell expansion. Decreased KEAP1 expression releases the block on the transcriptional activity of NRF2, resulting in increased expression of oxidative stress enzymes and proteins that favor cancer cells survival and proliferation [33, 50]. KLHL20 is an important KLHL family member related to cancer progression. KLHL20 is induced by HIF-1α protein and forms a KLHL20-cullin 3 complex that degrades the promyelocytic leukemia protein (PML) leading to prostate cancer progression . Mutations in ENC1 (KLHL37), also called NRP/B, are associated with brain tumors. Mutations reported in KLHL37 are primarily located in the Kelch domain but also exist in the BTB and BACK domains . KLHL37 protein is normally expressed in neurons but not in astrocytes; however, brain tumor cells that arise from astrocytes express KLHL37. It is hypothesized that mutated KLHL37 promotes cell growth, prevents apoptosis, and alters the cytoskeleton .
There are 42 KLHL family members encoded in the human genome, containing conserved BTB, BACK, and Kelch domains. KLHL proteins are known to be involved in the ubiquitination process, but the specific roles for each family member have not yet been elucidated. KLHL proteins will likely have multiple substrates. KEAP1 (KLHL19), for example, has at least three (NRF2, IKKβ and BCL-2) as reviewed in Tian et al. . Similar protein motifs amongst substrates such as the ETGE region in NRF2 and IKKβ could be one way to explain how a KLHL protein can bind to more than one protein . Another potential source of substrate diversity is possible through the BTB domain. BTB cross-dimerization between different KLHL proteins could theoretically allow for differential substrate binding depending on the spatial and temporal expression of KLHL proteins. Mutations in certain KLHL genes are detrimental and result in either Mendelian disease or human cancers. We anticipate that further studies will reveal that most, if not all, KLHL proteins have fundamental impacts on human biological processes and disease.
- Koonin EV, Senkevich TG, Chernos VI: A family of DNA virus genes that consists of fused portions of unrelated cellular genes. Trends Biochem Sci. 1992, 17: 213-214.View ArticlePubMedGoogle Scholar
- Zollman S, Godt D, Prive GG, Couderc JL, Laski FA: The BTB domain, found primarily in zinc finger proteins, defines an evolutionarily conserved family that includes several developmentally regulated genes in Drosophila. Proc Natl Acad Sci USA. 1994, 91: 10717-10721.PubMed CentralView ArticlePubMedGoogle Scholar
- Siggs OM, Beutler B: The BTB-ZF transcription factors. Cell Cycle. 2012, 11: 3358-3369.PubMed CentralView ArticlePubMedGoogle Scholar
- Bardwell VJ, Treisman R: The POZ domain: a conserved protein-protein interaction motif. Genes Dev. 1994, 8: 1664-1677.View ArticlePubMedGoogle Scholar
- Albagli O, Dhordain P, Deweindt C, Lecocq G, Leprince D: The BTB/POZ domain: a new protein-protein interaction motif common to DNA- and actin-binding proteins. Cell Growth Differ. 1995, 6: 1193-1198.PubMedGoogle Scholar
- Perez-Torrado R, Yamada D, Defossez PA: Born to bind: the BTB protein-protein interaction domain. Bioessays. 2006, 28: 1194-1202.View ArticlePubMedGoogle Scholar
- Kang MI, Kobayashi A, Wakabayashi N, Kim SG, Yamamoto M: Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes. Proc Natl Acad Sci USA. 2004, 101: 2046-2051.PubMed CentralView ArticlePubMedGoogle Scholar
- Minor DL, Lin YF, Mobley BC, Avelar A, Jan YN, Berger JM: The polar T1 interface is linked to conformational changes that open the voltage-gated potassium channel. Cell. 2000, 102: 657-670.View ArticlePubMedGoogle Scholar
- Melnick A, Ahmad KF, Arai S, Polinger A, Ball H, Borden KL, Carlile GW, Prive GG, Licht JD: In-depth mutational analysis of the promyelocytic leukemia zinc finger BTB/POZ domain reveals motifs and residues required for biological and transcriptional functions. Mol Cell Biol. 2000, 20: 6550-6567.PubMed CentralView ArticlePubMedGoogle Scholar
- Furukawa M, He YJ, Borchers C, Xiong Y: Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases. Nat Cell Biol. 2003, 5: 1001-1007.View ArticlePubMedGoogle Scholar
- Xu L, Wei Y, Reboul J, Vaglio P, Shin TH, Vidal M, Elledge SJ, Harper JW: BTB proteins are substrate-specific adaptors in an SCF-like modular ubiquitin ligase containing CUL-3. Nature. 2003, 425: 316-321.View ArticlePubMedGoogle Scholar
- Adams J, Kelso R, Cooley L: The kelch repeat superfamily of proteins: propellers of cell function. Trends Cell Biol. 2000, 10: 17-24.View ArticlePubMedGoogle Scholar
- Stogios PJ, Prive GG: The BACK domain in BTB-kelch proteins. Trends Biochem Sci. 2004, 29: 634-637.View ArticlePubMedGoogle Scholar
- Bomont P, Cavalier L, Blondeau F, Ben Hamida C, Belal S, Tazir M, Demir E, Topaloglu H, Korinthenberg R, Tüysüz B, Landrieu P, Hentati F, Koenig M: The gene encoding gigaxonin, a new member of the cytoskeletal BTB/kelch repeat family, is mutated in giant axonal neuropathy. Nat Genet. 2000, 26: 370-374.View ArticlePubMedGoogle Scholar
- Liang XQ, Avraham HK, Jiang S, Avraham S: Genetic alterations of the NRP/B gene are associated with human brain tumors. Oncogene. 2004, 23: 5890-5900.View ArticlePubMedGoogle Scholar
- Yang Y, Allen E, Ding J, Wang W: Giant axonal neuropathy. Cell Mol Life Sci: CMLS. 2007, 64: 601-609.View ArticlePubMedGoogle Scholar
- Shibata T, Kokubu A, Gotoh M, Ojima H, Ohta T, Yamamoto M, Hirohashi S: Genetic alteration of Keap1 confers constitutive Nrf2 activation and resistance to chemotherapy in gallbladder cancer. Gastroenterology. 2008, 135: 1358-1368. 1368 e1351-1354View ArticlePubMedGoogle Scholar
- Friedman JS, Ray JW, Waseem N, Johnson K, Brooks MJ, Hugosson T, Breuer D, Branham KE, Krauth DS, Bowne SJ, Sullivan LS, Ponjavic V, Gränse L, Khanna R, Trager EH, Gieser LM, Hughbanks-Wheaton D, Cojocaru RI, Ghiasvand NM, Chakarova CF, Abrahamson M, Göring HH, Webster AR, Birch DG, Abecasis GR, Fann Y, Bhattacharya SS, Daiger SP, Heckenlively JR, Andréasson S: Mutations in a BTB-Kelch protein, KLHL7, cause autosomal-dominant retinitis pigmentosa. Am J Hum Genet. 2009, 84: 792-800.PubMed CentralView ArticlePubMedGoogle Scholar
- Louis-Dit-Picard H, Barc J, Trujillano D, Miserey-Lenkei S, Bouatia-Naji N, Pylypenko O, Beaurain G, Bonnefond A, Sand O, Simian C, Vidal-Petiot E, Soukaseum C, Mandet C, Broux F, Chabre O, Delahousse M, Esnault V, Fiquet B, Houillier P, Bagnis CI, Koenig J, Konrad M, Landais P, Mourani C, Niaudet P, Probst V, Thauvin C, Unwin RJ, Soroka SD, Ehret G: KLHL3 mutations cause familial hyperkalemic hypertension by impairing ion transport in the distal nephron. Nat Genet. 2012, 44: 456-460. S451-453View ArticlePubMedGoogle Scholar
- MyHits. [http://myhits.isb-sib.ch]
- National Center for Biotechnology Information (NCBI). [http://www.ncbi.nlm.nih.gov/protein]
- European Bioinformatics Institute (EBI). [http://www.ebi.ac.uk/Tools/msa/clustalo]
- Bioinformatics Services. [http://www.ebi.ac.uk/Tools/phylogeny/clustalw2_phylogeny]
- Itoh K, Chiba T, Takahashi S, Ishii T, Igarashi K, Katoh Y, Oyake T, Hayashi N, Satoh K, Hatayama I, Yamamoto M, Nabeshima Y: An Nrf2/small Maf heterodimer mediates the induction of phase II detoxifying enzyme genes through antioxidant response elements. Biochem Biophys Res Commun. 1997, 236: 313-322.View ArticlePubMedGoogle Scholar
- Itoh K, Wakabayashi N, Katoh Y, Ishii T, Igarashi K, Engel JD, Yamamoto M: Keap1 represses nuclear activation of antioxidant responsive elements by Nrf2 through binding to the amino-terminal Neh2 domain. Genes Dev. 1999, 13: 76-86.PubMed CentralView ArticlePubMedGoogle Scholar
- Motohashi H, Yamamoto M: Carcinogenesis and transcriptional regulation through Maf recognition elements. Cancer Sci. 2007, 98: 135-139.View ArticlePubMedGoogle Scholar
- Kobayashi M, Itoh K, Suzuki T, Osanai H, Nishikawa K, Katoh Y, Takagi Y, Yamamoto M: Identification of the interactive interface and phylogenic conservation of the Nrf2-Keap1 system. Genes Cells. 2002, 7: 807-820.View ArticlePubMedGoogle Scholar
- Kobayashi A, Kang MI, Okawa H, Ohtsuji M, Zenke Y, Chiba T, Igarashi K, Yamamoto M: Oxidative stress sensor Keap1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf2. Mol Cell Biol. 2004, 24: 7130-7139.PubMed CentralView ArticlePubMedGoogle Scholar
- Furukawa M, Xiong Y: BTB protein Keap1 targets antioxidant transcription factor Nrf2 for ubiquitination by the Cullin 3-Roc1 ligase. Mol Cell Biol. 2005, 25: 162-171.PubMed CentralView ArticlePubMedGoogle Scholar
- McMahon M, Itoh K, Yamamoto M, Hayes JD: Keap1-dependent proteasomal degradation of transcription factor Nrf2 contributes to the negative regulation of antioxidant response element-driven gene expression. J Biol Chem. 2003, 278: 21592-21600.View ArticlePubMedGoogle Scholar
- Watai Y, Kobayashi A, Nagase H, Mizukami M, McEvoy J, Singer JD, Itoh K, Yamamoto M: Subcellular localization and cytoplasmic complex status of endogenous Keap1. Genes Cells. 2007, 12: 1163-1178.View ArticlePubMedGoogle Scholar
- Lo SC, Hannink M: PGAM5, a Bcl-XL-interacting protein, is a novel substrate for the redox-regulated Keap1-dependent ubiquitin ligase complex. J Biol Chem. 2006, 281: 37893-37903.View ArticlePubMedGoogle Scholar
- Mitsuishi Y, Motohashi H, Yamamoto M: The Keap1-Nrf2 system in cancers: stress response and anabolic metabolism. Frontiers in oncology. 2012, 2: 200-PubMed CentralView ArticlePubMedGoogle Scholar
- Padmanabhan B, Tong KI, Ohta T, Nakamura Y, Scharlock M, Ohtsuji M, Kang MI, Kobayashi A, Yokoyama S, Yamamoto M: Structural basis for defects of Keap1 activity provoked by its point mutations in lung cancer. Mol Cell. 2006, 21: 689-700.View ArticlePubMedGoogle Scholar
- Singh A, Misra V, Thimmulappa RK, Lee H, Ames S, Hoque MO, Herman JG, Baylin SB, Sidransky D, Gabrielson E, Brock MV, Biswal S: Dysfunctional KEAP1-NRF2 interaction in non-small-cell lung cancer. PLoS Med. 2006, 3: e420-PubMed CentralView ArticlePubMedGoogle Scholar
- Soltysik-Espanola M, Rogers RA, Jiang S, Kim TA, Gaedigk R, White RA, Avraham H, Avraham S: Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain. Mol Biol Cell. 1999, 10: 2361-2375.PubMed CentralView ArticlePubMedGoogle Scholar
- Williams SK, Spence HJ, Rodgers RR, Ozanne BW, Fitzgerald U, Barnett SC: Role of Mayven, a kelch-related protein in oligodendrocyte process formation. J Neurosci Res. 2005, 81: 622-631.View ArticlePubMedGoogle Scholar
- Jiang S, Avraham HK, Park SY, Kim TA, Bu X, Seng S, Avraham S: Process elongation of oligodendrocytes is promoted by the Kelch-related actin-binding protein Mayven. J Neurochem. 2005, 92: 1191-1203.View ArticlePubMedGoogle Scholar
- Montague P, Kennedy PG, Barnett SC: Subcellular localization of Mayven following expression of wild type and mutant EGFP tagged cDNAs. BMC Neurosci. 2010, 11: 63-PubMed CentralView ArticlePubMedGoogle Scholar
- Korutla L, Wang P, Jackson TG, Mackler SA: NAC1, a POZ/BTB protein that functions as a corepressor. Neurochem Int. 2009, 54: 245-252.View ArticlePubMedGoogle Scholar
- Tseng LA, Bixby JL: Interaction of an intracellular pentraxin with a BTB-Kelch protein is associated with ubiquitylation, aggregation and neuronal apoptosis. Mol Cell Neurosci. 2011, 47: 254-264.PubMed CentralView ArticlePubMedGoogle Scholar
- Hugosson T, Friedman JS, Ponjavic V, Abrahamson M, Swaroop A, Andréasson S: Phenotype associated with mutation in the recently identified autosomal dominant retinitis pigmentosa KLHL7 gene. Arch Ophthalmol. 2010, 128: 772-778.View ArticlePubMedGoogle Scholar
- Wen Y, Locke KG, Klein M, Bowne SJ, Sullivan LS, Ray JW, Daiger SP, Birch DG, Hughbanks-Wheaton DK: Phenotypic characterization of 3 families with autosomal dominant retinitis pigmentosa due to mutations in KLHL7. Arch Ophthalmol. 2011, 129: 1475-1482.PubMed CentralView ArticlePubMedGoogle Scholar
- Kigoshi Y, Tsuruta F, Chiba T: Ubiquitin ligase activity of Cul3-KLHL7 protein is attenuated by autosomal dominant retinitis pigmentosa causative mutation. J Biol Chem. 2011, 286: 33613-33621.PubMed CentralView ArticlePubMedGoogle Scholar
- Uchida K, Akita Y, Matsuo K, Fujiwara S, Nakagawa A, Kazaoka Y, Hachiya H, Naganawa Y, Oh-Iwa I, Ohura K, Saga S, Kawai T, Matsumoto Y, Shimozato K, Kozaki K: Identification of specific autoantigens in Sjogren's syndrome by SEREX. Immunology. 2005, 116: 53-63.PubMed CentralView ArticlePubMedGoogle Scholar
- Cirak S, von Deimling F, Sachdev S, Errington WJ, Herrmann R, Bönnemann C, Brockmann K, Hinderlich S, Lindner TH, Steinbrecher A, Hoffmann K, Privé GG, Hannink M, Nürnberg P, Voit T: Kelch-like homologue 9 mutation is associated with an early onset autosomal dominant distal myopathy. Brain. 2010, 133: 2123-2135.PubMed CentralView ArticlePubMedGoogle Scholar
- Xu M, Da YW, Liu L, Wang F, Jia JP: Giant axonal neuropathy caused by a novel compound heterozygous mutation in the gigaxonin gene. J Child Neurol. 2012, 10.1177/0883073812467688.Google Scholar
- Kroll J, Shi X, Caprioli A, Liu HH, Waskow C, Lin KM, Miyazaki T, Rodewald HR, Sato TN: The BTB-kelch protein KLHL6 is involved in B-lymphocyte antigen receptor signaling and germinal center formation. Mol Cell Biol. 2005, 25: 8531-8540.PubMed CentralView ArticlePubMedGoogle Scholar
- Puente XS, Pinyol M, Quesada V, Conde L, Ordóñez GR, Villamor N, Escaramis G, Jares P, Beà S, González-Díaz M, Bassaganyas L, Baumann T, Juan M, López-Guerra M, Colomer D, Tubío JM, López C, Navarro A, Tornador C, Aymerich M, Rozman M, Hernández JM, Puente DA, Freije JM, Velasco G, Gutiérrez-Fernández A, Costa D, Carrió A, Guijarro S, Enjuanes A: Whole-genome sequencing identifies recurrent mutations in chronic lymphocytic leukaemia. Nature. 2011, 475: 101-105.PubMed CentralView ArticlePubMedGoogle Scholar
- Ohta T, Iijima K, Miyamoto M, Nakahara I, Tanaka H, Ohtsuji M, Suzuki T, Kobayashi A, Yokota J, Sakiyama T, Shibata T, Yamamoto M, Hirohashi S: Loss of Keap1 function activates Nrf2 and provides advantages for lung cancer cell growth. Cancer Res. 2008, 68: 1303-1309.View ArticlePubMedGoogle Scholar
- Yuan WC, Lee YR, Huang SF, Lin YM, Chen TY, Chung HC, Tsai CH, Chen HY, Chiang CT, Lai CK, Lu LT, Chen CH, Gu DL, Pu YS, Jou YS, Lu KP, Hsiao PW, Shih HM, Chen RH: A Cullin3-KLHL20 Ubiquitin ligase-dependent pathway targets PML to potentiate HIF-1 signaling and prostate cancer progression. Cancer Cell. 2011, 20: 214-228.View ArticlePubMedGoogle Scholar
- Tian H, Zhang B, Di J, Jiang G, Chen F, Li H, Li L, Pei D, Zheng J: Keap1: one stone kills three birds Nrf2, IKKbeta and Bcl-2/Bcl-xL. Cancer Lett. 2012, 325: 26-34.View ArticlePubMedGoogle Scholar
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