Copy number variation in CEP57L1 predisposes to congenital absence of bilateral ACL and PCL ligaments

Table 2 qPCR validation primer information for EBV cells genomic DNA

Gene name	Gene	Amplicon position^a	UPL probe #	Left primer^b	Right primer^b
CEP57L1	Centrosomal protein 57 kDa-like 1	Amplicon located in intronic genomic DNA between exons 6 and 7 (chr6:109474636-109474742) chr6:109474636-109474742	75	gaggggtcccgttatgttg	gcgtggtggctcatacttg
GAPDH	Glyceraldehyde 3-phosphate dehydrogenase	chr12: 6645563-6645625	10	gctgcattcgccctctta	gaggctcctccagaatatgtga
SNCA	Synuclein, alpha	chr4: 90743466-90743537	68	gctgagaagaccaaagagcaa	ctgggctactgctgtcacac

^aAmplicon position as reported by UCSC genome browser (hg19) in silico PCR tool
^bAll primers are listed 5′ to 3′

ISSN: 1479-7364