Fig. 7

STAT3 pY705 levels as a marker for JAK/STAT signaling in CI patients. A Dot plot depicts STAT3 pY705 measured by flow cytometry in subcellular PBMCs. Line bar represents mean. Control samples were unstimulated or stimulated for 15 min with 400 μl Combo (cytokines (100 ng/ml), LPS (10 μg/ml; Sigma-Aldrich) or PMA (100 ng/ml; Sigma-Aldrich)). Healthy and CI patient samples were unstimulated by any substances. Results are plotted in dot plot showing median with interquartile range and statistical significance as *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. B Dot plot depicts STAT3 pY705 analyzed across the 4 CI dysbiosis clusters (P2, P3, P4, and P5) compared with healthy controls (P1). Results are plotted in dot plot showing median with interquartile range and SEM statistical significance as *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001