Fig. 1
From: GEN1 as a risk factor for human congenital anomalies of the kidney and urinary tract
Stability of point-mutated GEN1 proteins. The protein stability was measured by the rate of wild-type and mutant protein degradation under the same conditions, and the stability results of all mutated proteins were evaluated from the baseline results of wild-type proteins. (A) Western blot analysis of point-mutant proteins and wild-type GEN1 protein treated with cycloheximide at 0, 4, 8, 12, 24, and 30 h. The grayscale bands represent the amount of protein involved. * sites in the non-CAKUT group. The left side band in Figure A is the inner reference GAPDH, and the right band is the wild-type and mutant protein displayed using the FLAG label. (B) Grayscale chart of the western blot analysis. The results are reported as mean ± SEM for three individual experiments. * sites in the non-CAKUT group. Abbreviations: SEM, standard error of the mean