Figure 5From: Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environmentGenotyping of NATI SNPs using the MGB Eclipse System. Melt curves analysis of a) A-T alleles and b) A/C alleles. Fa is fluorescein and Fb is tetrachlorofluorescein. Alleles are underlined in the probe sequences. Small case letters represent modified bases. The x-axis and y-axis represent the temperature in °C and the first derivative, respectively.Back to article page