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Figure 6 | Human Genomics

Figure 6

From: Single nucleotide polymorphism genotyping by two colour melting curve analysis using the MGB Eclipse™ Probe System in challenging sequence environment

Figure 6

a) A portion of the sequence of the RAD23B-02 SNP (gi:12871592). The SNP is shown in parenthesis. Primer sequences are underlined. b) and c) MGB Eclipse data with two probes labelled with fluorescein specific for the A-allele and 5'-tetrachloro-fluorescein specific for the G-allele, respectively. The alleles are underlined in the probe sequences. 'g' is modified G. The x-axis and y-axis represent the temperature in °C and the first derivative, respectively.

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Human Genomics

ISSN: 1479-7364