The pharmacokinetics of midazolam in CYP3A4-humanised mice. The graphs show the time course of the serum concentrations of (A) midazolam and (B) 1' -hydroxymidazolam (1' -OH-MDZ) from wild-type (WT), CYP3A4-humanised mice (Tg-CYP3A4) after one single oral administration of midazolam. Venous blood was obtained 0, 0.5, 1, 2, 4, 6, 8, 12 and 24 hours after administration. Values represent the mean and the vertical lines the standard errors of the mean of midazolam and 1" -OH-MDZ from three mice. Note the higher maximal concentration (Cmax) for midazolam in WT mice compared with Tg-CYP3A4 mice.