Figure 1
From: Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung
Schematic representation of the variable and conserved regions of the I6S rRNA genes, using Escherichia coli rrsA (ECDHI0B_4040) as a reference. The diagram illustrates the approximate positions of nine variable (V) regions that are interspersed with conserved regions. LH-PCR primer sequences for the conserved regions are included. The 8 F, 112 F/R, 338 F/R, 518 F/R and 785 F/R primers have also been referred to as 27 IF P2, 355 F/R, 536 F/R and 802 F/R, respectively. F and R refer to forward and reverse, respectively. The degenerate nucleotides M, R, S, Wand Y stand for A/C, A/G, G/C, A/T and C/T, respectively.