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Table 1 Primer sequences used for the mutational analysis of β-catenin and APC genes in the Wnt pathway

From: Analysis of molecular aberrations of Wnt pathway gladiators in colorectal cancer in the Kashmiri population

Gene

Amplicon

Primer sequence

Amplicon size (bp)

Annealing temperature (°C)

β-catenin

Exon 3

BCat F: 5'-ATG GAA CCA GAC AGA AAA GC-3'

BCat R: 5'-GCT ACT TGT TCT TGA GTG AAG-3'

200

58

APC

Exon 15

APC A F: 5'-CAGACTTATTGTGTAGAAGA- 3'

APC A R: 5'-ATCCTGAAGAAAATTCAACA-3'

295 for codons

1260 to 1359

52

  

APC B F: 5'-AGGGTTCTAGTTTATCTTCA-3'

APC B R: 5'-TCTGCTTGGTGGCATGGTTT-3'

293 for codons

1339 to 1436

55

  

APC C F: 5'-GGCATTATAAGCCCCAGTGA-3'

APC C R: 5'-AAATGGCTCATCGAGGCTCA-3'

290 for codons

1417 to 1516

55

  

APC D F: 5'-ACTCCAGATGGATTTTCTTG-3'

APC D R: 5'-GGCTGGCTTTTTTGCTTTAC-3'

300 for codons

1497 to 1596

55