Gene | Amplicon | Primer sequence | Amplicon size (bp) | Annealing temperature (°C) |
---|---|---|---|---|
β-catenin | Exon 3 | BCat F: 5'-ATG GAA CCA GAC AGA AAA GC-3' BCat R: 5'-GCT ACT TGT TCT TGA GTG AAG-3' | 200 | 58 |
APC | Exon 15 | APC A F: 5'-CAGACTTATTGTGTAGAAGA- 3' APC A R: 5'-ATCCTGAAGAAAATTCAACA-3' | 295 for codons 1260 to 1359 | 52 |
APC B F: 5'-AGGGTTCTAGTTTATCTTCA-3' APC B R: 5'-TCTGCTTGGTGGCATGGTTT-3' | 293 for codons 1339 to 1436 | 55 | ||
APC C F: 5'-GGCATTATAAGCCCCAGTGA-3' APC C R: 5'-AAATGGCTCATCGAGGCTCA-3' | 290 for codons 1417 to 1516 | 55 | ||
APC D F: 5'-ACTCCAGATGGATTTTCTTG-3' APC D R: 5'-GGCTGGCTTTTTTGCTTTAC-3' | 300 for codons 1497 to 1596 | 55 |