Table 2 Primer sequences used for hypermethylation analysis of the promoter region of the APC gene
Gene | Amplicon | Primer sequence | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
APC | 1A | APC 1A UF: 5'-TGTTTTATTGTGGAGTGTGGGTT-3' | 108 | 60 |
Promoter | APC 1A UR: 5'-CCAATCAACAAACTCCCAACAA-3' | |||
APC 1A MF: 5'-TATTGCGGAGTGCGGGTC-3' APC 1A MR: 5'-TCGACGAACTCCCGACGA-3' | 98 | 55 | ||
APC | 1B | APC 1B UF: 5'-GATAGAATAGTGAATGAGTGTTT-3' | 195 | 55 |
Promoter | APC 1B UR: 5'-CTTCCAACAACCACACCCCA-3' | |||
APC 1B MF: 5'-TAGAATAGCGAACGAGTGTTC-3' APC 1B MR: 5'-TCCGACGACCACACCCCG-3' | 190 | 55 |