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Table 2 Primer sequences used for hypermethylation analysis of the promoter region of the APC gene

From: Analysis of molecular aberrations of Wnt pathway gladiators in colorectal cancer in the Kashmiri population

Gene Amplicon Primer sequence Amplicon size (bp) Annealing temperature (°C)
APC 1A APC 1A UF: 5'-TGTTTTATTGTGGAGTGTGGGTT-3' 108 60
  Promoter APC 1A UR: 5'-CCAATCAACAAACTCCCAACAA-3'   
   APC 1A MF: 5'-TATTGCGGAGTGCGGGTC-3'
APC 1A MR: 5'-TCGACGAACTCCCGACGA-3'
98 55
APC 1B APC 1B UF: 5'-GATAGAATAGTGAATGAGTGTTT-3' 195 55
  Promoter APC 1B UR: 5'-CTTCCAACAACCACACCCCA-3'   
   APC 1B MF: 5'-TAGAATAGCGAACGAGTGTTC-3'
APC 1B MR: 5'-TCCGACGACCACACCCCG-3'
190 55