Table 2 Primers for CYP2E1 gene polymorphism
From: Role of CYP2E1 genotypes in susceptibility to colorectal cancer in the Kashmiri population
Target codon | Sequence | Amplicon (bp) | Tm (°C) |
|---|---|---|---|
CYP2E1 * 5B | F5'-CCAGTCGAGTCTACATTGTCA-3' | 413 bp | 55 |
RsaI | R5'-TTCATTCTGTCTTCTAACTGG-3' | Â | Â |
CYP2E1 | F5'-GTGATGGAAGCCTGAAGAACA-3' | 729 bp for insertion | 66 |
96-bp insertion | R5'-CTTTGGTGGGGTGAGAACAG-3' | 633 bp for non-insertion | Â |