Figure 3

Functional analyses of MDM2 promoter haplotype H1- and H2-derived mutations in HeLa cells. H1- and H2-derived constructs carrying mutations introduced by site-directed mutagenesis and tested for luciferase gene reporter assays in pGL3 basic vector (left panel). Relative luciferase activity of the H1- and H2-derived promoter haplotypes was measured following transient transfection in HeLa cells (right panel). The empty promoterless pGL3 basic vector was used as negative control. Results are expressed in a ratio of Firefly/Renilla activity multiplied by 100. Promoter haplotype H2 was used as reference against which relative expression was compared. The p values are calculated from four replicates with unpaired student’s t test. The p value between H1 and H2 is 0.0008.