Figure 3
Validation of protein expression data by qPCR analysis. (A) Genes expressed at higher levels in dorsal iris. (B) Genes expressed at higher levels during regeneration. (C) Genes expressed at higher levels in the dorsal iris and during regeneration. (D) Gene expressed at higher levels in the ventral iris and during regeneration. (E) Gene expressed at higher levels in the ventral iris. (F) Gene expressed at higher levels in the intact iris. Student’s t-test for independent samples was used for statistical significance. Homoscedasticity was assumed when Levene’s test p value was greater than 0.05. Asterisk (*) indicates statistical significance (Student’s t-test; p < 0.05). Each bar represents the average of triplicate values. Error bars represent standard deviation. Lines on the top of the graph compare samples during regeneration and control. Lines on the top of bars corresponding to a single day compare dorsal and ventral iris. For simplicity, only the statistics relevant for each group are presented on the graphs.