Gene expression comparison of different regenerating tissues from amphibians reveals a canonical regeneration program. Proteins found to be up-regulated during regeneration in the dorsal iris in the present study were compared to gene expression datasets related to amphibian regeneration published previously. Datasets included gene expression profiles from DNA microarray analysis of newt brain, spinal cord, hindlimbs, dorsal iris, heart, forelimbs and tail regeneration, microarray and RNA-seq analysis from axolotl limb regeneration, and LC-MS/MS from newt heart and axolotl limb regeneration. Newts and axolotls are presented with black color. Respective regenerating organs are colored white on the animals. The central grey column depicts the collection of all the gene expression data from the different regenerating tissues located at the periphery. The node and the three arrows represent the result of the data comparison. Boxes highlight the three major events of the common canonical regeneration program that was identified.