Fig. 3From: SIRT1 affects DNA methylation of polycomb group protein target genes, a hotspot of the epigenetic shift observed in ageingMeasurement of mRNAs for PRC components, KDM2B and DNMTs under conditions of SIRT1 knockdown. Data are for HuVECs (a) and Caco-2 cells (b). SIRT1 knockdown was achieved using two different siRNAs, as indicated. Measurements were by RT-qPCR. Data are mean ± SEM for n = 6–15 relative to the reference genes GAPDH and TOP1 and normalised according to the measurements for the control siRNA. *P < 0.05; **P < 0.01; ***P < 0.001 compared with control by one-way ANOVA then Dunnett’s post hoc testBack to article page