Table 1 The list of genotyping primers for six CpG-SNPs
From: Association of six CpG-SNPs in the inflammation-related genes with coronary heart disease
Gene | SNP | Primers | Sequence (5′ to 3′) |
|---|---|---|---|
IL1B | rs16944 | 1st PCR primer | ACGTTGGATGAGAGGCTCCTGCAATTGACA |
| Â | Â | 2nd primer | ACGTTGGATGCTGTCTGTATTGAGGGTGTG |
| Â | Â | Extend primer | GGGGTGGGTGCTGTTCTCTGCCTC |
IL1R2 | rs2071008 | 1st PCR primer | ACGTTGGATGGAAAAATCCATGCAGCCTCC |
| Â | Â | 2nd primer | ACGTTGGATGTGGTGGCTGACTTTCCAAGG |
| Â | Â | Extend primer | TGGGAAGAAGCAAGCACCCC |
PLA2G7 | rs9395208 | 1st PCR primer | ACGTTGGATGTGGACCCGCGGTTAACTTAG |
| Â | Â | 2nd primer | ACGTTGGATGATCAGGTCTGCGGAAAGGAG |
| Â | Â | Extend primer | GCATTGCCTGGCTCT |
FAM5C | rs12732361 | 1st PCR primer | ACGTTGGATGTTACACAGAGAGCCACGAAC |
| Â | Â | 2nd primer | ACGTTGGATGAGGATCACCACGAATCACCC |
| Â | Â | Extend primer | GAAACCCCCACCATTCCCCA |
CD40 | rs1800686 | 1st PCR primer | ACGTTGGATGATGGATGGGAAGTTGAGACG |
| Â | Â | 2nd primer | ACGTTGGATGCCCAACTCAGAATTTCGCTC |
| Â | Â | Extend primer | GTCGCTTTCAAAGGAAATTCCCT |
CD36 | rs2065666 | 1st PCR primer | ACGTTGGATGCTCTGAAGATATAATGACAAG |
| Â | Â | 2nd primer | ACGTTGGATGCAGTTTCTCTGTTCACTTCG |
| Â | Â | Extend primer | CGTTCACTTCGTTTTAGTATAGAATTA |