Table 1 Sequence error rates for different NGS platforms in comparison with the traditional Sanger technology (updated as of 2012 at http://www.molecularecologist.com/next-gen-table-3c-2014/). See Glenn (2011) [41] for more details. Single-pass reads are those raw sequences that have not been subject to consensus adjustment incorporated in final base calling
Instrument | Primary error type | Single-pass error rate (%) | Final error rate (%) |
|---|---|---|---|
Sanger—ABI 3730XL capillary (benchmark) | Substitution | 0.1–1 | 0.1–1 |
454—all models | Indel | 1 | 1 |
Illumina—all models | Substitution | ~0.1 | ~0.1 |
Ion Torrent—all chips | Indel | ~1 | ~1 |
SOLiD—5500XL | A-T bias | ~5 | ≤0.1 |
Oxford Nanopore | Deletion | ≥4 | 4 |
PacBio RS | Indel | ~13 | ≤1 |