Table 5 The oligonucleotide primers for SNaPshot primer extension reactions
From: Multiplex SNaPshot—a new simple and efficient CYP2D6 and ADRB1 genotyping method
Multiplex pool | SNV | Primer direction | Peak to SNV correspondence | Primer length | Primer sequence (5′ → 3′) |
|---|---|---|---|---|---|
1 | rs28371725C/T | F(C/T) | C=C T=T | 16 | CCCCGCCTGTACCCTT |
rs1801252A/G | F(A/G) | A=A G=G | 18 | GACTCTCCCGCCAGCGAA | |
rs72549346-/AC | R(T/G) | T=AC C=- | 30 | GACTGACTGACTGCCGTGATTCATGAGGTG | |
rs16947A/G | F(A/G) | A=A G=G | 40 | GACTGACTGACTGACTGACTGAGGTCAGCCACCACTATGC | |
rs5030656-/CTT | F(T/C) | T=CTT C=- | 40 | GACTGACTGACTGACTGACTGATGGCAGCCACTCTCACCT | |
rs72549351-/AGTC | R(C/G) | C=AGTC G=- | 46 | GACTGACTGACTGACTGACTGACTGACTGACCCCCCCGAGACCTGA | |
rs72549353-/AGTT | F(T/A) | T=AGTT A=- | 46 | GACTGACTGACTGACTGACTGACTGACCAGGTCATCCTGTGCTCAG | |
rs35742686-/T | F(T/G) | T=T G=- | 52 | GACTGACTGACTGACTGACTGACTGACTGACTGACTGGGTCCCAGGTCATCC | |
rs5030867T/G | R(A/C) | A=T C=G | 52 | GACTGACTGACTGACTGACTGACTGACTGACTCCTCCTGCTCATGATCCTAC | |
2 | rs1801253C/G | F(C/G) | C=C G=G | 15 | CGCAAGGCCTTCCAG |
rs72549356-/GGGGCGAAAGGGGCGAAA | R(T/A) | T=GGGGCGAAAGGGGCGAAA A=- | 18 | GACTGCCCCTTTCGCCCC | |
rs1065852G/A | R(C/T) | C=G T=A | 36 | GACTGACTGACTGACTGACTCTGGGCTGCACGCTAC | |
rs3892097T/C | R(A/G) | A=T G=C | 36 | GACTGACTGACTGACTGACTGCATCTCCCACCCCCA | |
rs28371706G/A | R(C/T) | C=G T=A | 43 | GACTGACTGACTGACTGACTGACTGACTCGCCTGTGCCCATCA | |
rs5030862T/C | R(A/G) | A=T G=C | 43 | GACTGACTGACTGACTGACTGACTGACTCCCCTGCCACTGCCC | |
rs72549354-/C | F(T/C) | T=- C=C | 47 | GACTGACTGACTGACTGACTGACTGACTCGACTCCTCCTTCAGTCCC | |
rs5030655-/A | R(T/G) | T=T G=- | 51 | GACTGACTGACTGACTGACTGACTGACTGACTCAAGAAGTCGCTGGAGCAG | |
rs1135822A/T | F(A/T) | A=A T=T | 55 | GACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCATAGCGCGCCAGGA | |
rs5030865A/C/T | F(A/C/T) | A=A C=C T=T | 59 | TGACTGACTGACTGACTGACTGACTGACTGACTGACTGACTCTTCTGCCCATCACCCAC |