Fig. 4

Relative transcriptogram for NC-ADPKD/NK. The x-axis indicates the relative positon in the ordered list of 9684 genes. Upper panel the relative transcriptograms are presented as means ± s.e.m. with NC-ADPKD (blue line ± cyan region) and NK (black line ± gray region) given relative to NK. Center panel (shaded gray): P value from a two-tailed Weyl’s t test is plotted for each point of the transcriptograms. Horizontal lines mark P = 0.05 and P = 0.01. Location in the ordered list of Pkd1 and Pkd2, the causative genes for ADPKD, is shown. Lower panel Term enrichment for transcriptogram regions with significantly changed expression. A term enrichment value of 1 on the y-axis indicates that all genes in an interval of radius r = 30 participate in a given GO term or KEGG pathway. As an example of how to read the relative transcriptogram figures, NC-ADPKD cells have higher-than-normal gene expression in the region of the peak labeled ‘cell cycle’ in the upper panel with a significance of P < 0.01 (middle panel). This region is enriched with genes associated with ‘cell cycle’ KEGG terms and GO pathways (marked with a bar and identified by referring to the lower panel, which shows term enrichment)