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Table 1 Summary of RNA sequencing read mapping result

From: Transcriptome analysis of bronchoalveolar lavage fluid from children with severe Mycoplasma pneumoniae pneumonia reveals novel gene expression and immunodeficiency

Sample number Mild 202 Mild 224 Mild 247 Severe 177 Severe 223 Severe 324
Total reads 55492664 47186542 64792680 68897254 42136856 46318894
Total mapped 48535137 (87.46%) 40517410 (85.87%) 56013052 (86.45%) 60481397 (87.78%) 36565361 (86.78%) 40224088 (86.84%)
Multiple mapped 844736 (1.52%) 614681 (1.30%) 797662 (1.23%) 881129 (1.28%) 543196 (1.29%) 630869 (1.36%)
Uniquely mapped 47690401 (85.94%) 39902729 (84.56%) 55215390 (85.22%) 59600268 (86.51%) 36022165 (85.49%) 39593219 (85.48%)
Reads map to “+” 23844413 (42.97%) 19967766 (42.32%) 27594117 (42.59%) 29790936 (43.24%) 18008541 (42.74%) 19797319 (42.74%)
Reads map to “−” 23845988 (42.97%) 19934963 (42.25%) 27621273 (42.63%) 29809332 (43.27%) 18013624 (42.75%) 19795900 (42.74%)
Non-splice reads 30580304 (55.11%) 22475106 (47.63%) 34530918 (53.29%) 37577003 (54.54%) 20733762 (49.21%) 24205574 (52.26%)
Splice reads 17110097 (30.83%) 17427623 (36.93%) 20684472 (31.92%) 22023265 (31.97%) 15288403 (36.28%) 15387645 (33.22%)
  1. Total reads: The number of sequence after sequencing data filtering
  2. Total mapped: The number of sequence which can map to the genome
  3. Multiple mapped: The number of sequence which have multiple location on the reference sequencing
  4. Uniquely mapped: The number of sequence which have single location on the reference sequencing
  5. Reads map to “+”: The statistics of sequence that were mapped on the “+” chain of the genome
  6. Reads map to “−”: The statistics of sequence that were mapped on the “−” chain of the genome