Table 1 Summary of RNA sequencing read mapping result
Sample number | Mild 202 | Mild 224 | Mild 247 | Severe 177 | Severe 223 | Severe 324 |
|---|---|---|---|---|---|---|
Total reads | 55492664 | 47186542 | 64792680 | 68897254 | 42136856 | 46318894 |
Total mapped | 48535137 (87.46%) | 40517410 (85.87%) | 56013052 (86.45%) | 60481397 (87.78%) | 36565361 (86.78%) | 40224088 (86.84%) |
Multiple mapped | 844736 (1.52%) | 614681 (1.30%) | 797662 (1.23%) | 881129 (1.28%) | 543196 (1.29%) | 630869 (1.36%) |
Uniquely mapped | 47690401 (85.94%) | 39902729 (84.56%) | 55215390 (85.22%) | 59600268 (86.51%) | 36022165 (85.49%) | 39593219 (85.48%) |
Reads map to “+” | 23844413 (42.97%) | 19967766 (42.32%) | 27594117 (42.59%) | 29790936 (43.24%) | 18008541 (42.74%) | 19797319 (42.74%) |
Reads map to “−” | 23845988 (42.97%) | 19934963 (42.25%) | 27621273 (42.63%) | 29809332 (43.27%) | 18013624 (42.75%) | 19795900 (42.74%) |
Non-splice reads | 30580304 (55.11%) | 22475106 (47.63%) | 34530918 (53.29%) | 37577003 (54.54%) | 20733762 (49.21%) | 24205574 (52.26%) |
Splice reads | 17110097 (30.83%) | 17427623 (36.93%) | 20684472 (31.92%) | 22023265 (31.97%) | 15288403 (36.28%) | 15387645 (33.22%) |