Fig. 2
From: Identification of functional single nucleotide polymorphisms in the branchpoint site
CYP2C19 alternative splicing forms in minigene-transfected 293T and in EBV-transformed B cells carrying different genotypes at SNP rs12769205. CYP2C19 RT-PCR was performed with a 293T cells transfected with minigene of rs12769205, genotype A or G, using EGFP-F and CYP2C19 SalIR primers, and b cDNA from B cells carrying different genotypes AA, AG, and GG at rs12769205 position using CYP2C19 ex2F and CYP2C19 ex4R primers or c cDNA from B cells using CYP2C19 in2F and CYP2C19 ex4R as primer set. Marker represents the 100-bp DNA ladder and indicated 500-bp site. See Additional file 2: Table S2 for primer sequences