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Fig. 3 | Human Genomics

Fig. 3

From: Identification of functional single nucleotide polymorphisms in the branchpoint site

Fig. 3

PIP5KL1, KLC3, and MYH11 alternative splicing in minigene-transected 293T cell. RT-PCR was performed in minigene-transfected 293T cells. a PIP5KL1; rs13296170, genotype A or C, was using EGFP-F and PIP5KL1 KpnIR primers. b KLC3 (land 1~5); rs67785924, genotype ACCTC (Wt) or deletion (Del), with EGFP-F and KLC3 BamHIR primers, and MYH11 (land 7~11); rs75434223, genotype A or C, was using EGFP-F and MYH11 BamHIR primers. Marker represents the 100-bp DNA ladder and indicated 500-bp site. See Additional file 2: Table S2 for primer sequences

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