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Fig. 4 | Human Genomics

Fig. 4

From: Identification of functional single nucleotide polymorphisms in the branchpoint site

Fig. 4

Nucleotide sequences of KLC3 intron 12 and alternative splicing forms in minigene-transected 293T cell. a Nucleotide sequences at 3′part of the KLC3 intron 12. Possible splicing branchpoint adenine is indicated by bold and uppercase, SNP rs67785924 is underlined, and asterisk shown after deletion sequence. The following shown each A substituted minigene. b RT-PCR was performed in 293T cells transfected with different KLC3 adenine substitution minigene constructs: ACCTC (Wt); ACCTC deletion (Del); Wt with AG (AG form); Wt with GA (GA form); Wt with GG (GG form) and Del with GG form. Marker represents the 100-bp DNA ladder and indicated 500-bp site. See Additional file 2: Table S2 for primer sequences

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