Fig. 2
From: Evaluation of a bone morphogenetic protein 6 variant as a cause of iron loading
Cellular characterization of p.Q118dup BMP6 protein in CHO cells. a Representative immunofluorescence image of stably transfected CHO cells. The HA tag on the N-terminus of the pro-peptide, and the V5 tag on the C-terminus of the mature protein co-localize, and no difference in localization of the wildtype (WT) and p.Q118dup variant of BMP6 was observed. b Western blot identifying the full-length (FL) and mature BMP6 proteins in cell lysate (CP) and conditioned media (M) from untransfected (UT), wildtype (WT), and p.Q118dup (Q118dup) BMP6 transfected CHO cells. c Quantification of Western blot shown in b (n = 3). The ratio of mature proteins in conditioned media (M) to cell lysate (CP) shows no difference in protein secretion between WT and p.Q118dup BMP6 transfected cells