S. no. | Primer | Sequences (5′-3′) | Length (bp) | Reaction conditions |
---|---|---|---|---|
1 | K14 F | GTGGGCAGTGAGAAGGTGAC | 966 | Denaturation, 30 s at 94 °C Annealing, 45 s at 59 °C Extension, 60 s at 72 °C No. of cycles, 35 |
 | K14 R | AGAGGAGAACTGGGAGGAGG | ||
2 | K10 F | GGCTCATCAGGTGGCTAT | 812 | Denaturation, 30 s at 94 °C Annealing, 45 s at 58 °C Extension, 60 s at 72 °C No. of cycles, 35 |
 | K10 R | CAGGCTTCAGCATCTTTG | ||
3 | K16 F | GTGAAGATCCGTGACTGG | 856 | Denaturation, 30 s at 94 °C Annealing, 45 s at 56 °C Extension,60 s at 72 °C No. of cycles, 35 |
 | K16 R | TGCTGGGAGGAAAGGTGG | ||
4 | K17 F | CTTCCGCACCAAGTTTGA | 753 | Denaturation, 30 s at 94 °C Annealing, 45 s at 55 °C Extension, 60 s at 72 °C No. of cycles, 35 |
 | K17 R | TTGCCATCCTGGACCTCT |