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Table 1 Primer sequences and reaction conditions used for PCR

From: Mutational analysis of epidermal and hyperproliferative type I keratins in mild and moderate psoriasis vulgaris patients: a possible role in the pathogenesis of psoriasis along with disease severity

S. no.

Primer

Sequences (5′-3′)

Length (bp)

Reaction conditions

1

K14 F

GTGGGCAGTGAGAAGGTGAC

966

Denaturation, 30 s at 94 °C

Annealing, 45 s at 59 °C

Extension, 60 s at 72 °C

No. of cycles, 35

 

K14 R

AGAGGAGAACTGGGAGGAGG

2

K10 F

GGCTCATCAGGTGGCTAT

812

Denaturation, 30 s at 94 °C

Annealing, 45 s at 58 °C

Extension, 60 s at 72 °C

No. of cycles, 35

 

K10 R

CAGGCTTCAGCATCTTTG

3

K16 F

GTGAAGATCCGTGACTGG

856

Denaturation, 30 s at 94 °C

Annealing, 45 s at 56 °C

Extension,60 s at 72 °C

No. of cycles, 35

 

K16 R

TGCTGGGAGGAAAGGTGG

4

K17 F

CTTCCGCACCAAGTTTGA

753

Denaturation, 30 s at 94 °C

Annealing, 45 s at 55 °C

Extension, 60 s at 72 °C

No. of cycles, 35

 

K17 R

TTGCCATCCTGGACCTCT