Fig. 6
IPCR analysis of H2O2-induced chromosome breaks within the ABL gene in NP69 cells. a IPCR result obtained from H2O2-treated NP69 cells. NP69 cells were either untreated (lanes 2–7) or treated with 100 μM of H2O2 for 16 h (lanes 8–13) and 24 h (lanes 14–19). Genomic DNA was isolated and manipulated for nested IPCR. Double digestion with Age I and EcoR I was employed to eliminate competition of the intact fragments in the amplification process. Each cell sample consisted of six replicates (R1–6) in the nested IPCR. The IPCR products were analysed on 1.0% agarose gel. Side bracket indicates the possible IPCR bands derived from the ABL cleaved chromosome. Negative control for PCR was included (Lane 20). M: 100 bp DNA ladder. b The average number of DNA cleavage detected within the ABL gene. The data was expressed as means and SD of three independent experiments. Each experiment consisted of 1–3 sets of IPCR. Each set of IPCR was performed in 4–7 IPCR replicates for each cell sample. *p < 0.01, **p < 0.001 (Student’s t test)