Fig. 7
IPCR analysis of H2O2-induced chromosome breaks within the ABL gene in HK1 cells. a IPCR result obtained from H2O2-treated HK1 cells. HK1 cells were either untreated (lanes 2–7) or treated with 50 μM of H2O2 for 8 h (lanes 8–13). Genomic DNA was isolated and manipulated for nested IPCR. In the manipulation for nested IPCR, samples were subjected to double digestion with Age I and EcoR I to eliminate the competition of the intact fragments for amplification process. Each cell sample consisted of six replicates in nested IPCR. The IPCR products were analysed on 1.0% agarose gel. Side bracket indicates the possible IPCR bands derived from the ABL cleaved chromosome. Negative control for PCR was included (lane 14). M: 100 bp DNA ladder. b The average number of DNA cleavage detected within the ABL gene. The data was expressed as means and SD of three independent experiments. Each experiment consisted of 1–3 sets of IPCR. Each set of IPCR was performed in 6 IPCR replicates for each cell sample. *Pp< 0.001 (Student’s t test)