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Fig. 9 | Human Genomics

Fig. 9

From: Oxidative stress-induced chromosome breaks within the ABL gene: a model for chromosome rearrangement in nasopharyngeal carcinoma

Fig. 9

Shift translocations detected in H2O2-treated NP69 cells. a Treatment of NP69 with 100 μM of H2O2 for 16 h resulted in shift translocation. The DNA sequences 1–184 and 413–998 (without the box) represent the sequence derived from the ABL gene. The DNA sequence 185–412 (within the box) represents the sequence derived from the LHFPL3 gene which locates at chromosome 7. Region of microhomology (185–188, TGCC) was found at the breakpoint junctions. The translocated fragment (228 bp) of LHFPL3 gene is corresponding to coordinates 108,006–108,234 [Ensembl:ENSG00000187416]. b Treatment of NP69 with 10 μM of H2O2 for 24 h resulted in shift translocation. The DNA sequences 1–524 and 672–742 (without the box) represent the sequence derived from the ABL gene. The DNA sequence 525–671 (within the box) represents the sequence of the fragment translocated to the ABL gene. This translocated fragment (147 bp) is derived from chromosome 5. The disabled homologue 2 (DAB) gene is 1,263,556 bp at the 5′ end of this translocated fragment while a gene encoding for a hypothetical protein is 22,122 bp at the 3′ end

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