Fig. 4
From: A novel LRAT mutation affecting splicing in a family with early onset retinitis pigmentosa
Results of in silico splice prediction tools. Lowercase nucleotides indicate intron 2 sequences, upper case nucleotides indicate exon 3 sequences, and the fragment highlighted in blue is the coding region. Nucleotides marked in red indicate potential splice donor or acceptor sites. The YAG sequence is shown in yellow, the polypyrimidine tract (PPT) is shown in green, and the branch point sequence (BPS) is shown in purple. The scores calculated for wildtype sequence (WT) and mutated sequence (Mut) using Human Splice Finder, SpliceView, BDGP, and NetGene2 are displayed below each splice site. A higher score implies greater potential for a splice site. NA means no splice site predictions above threshold of the in silico tool