Fig. 5

SNV mutations in N-T-M trio samples. a Genotypic changes in T- or M-stage sequences. The numbers of genotypic changes in T- or M-stage sequences relative to N-stage sequences in each of the five case groups are represented by ΔTN and ΔMN, respectively. The five case groups include AluScan group of 2 N-T-M trio sets analyzed with AluScan sequencing, WGS-Liver-M group of 4 trio sets of liver-to-lung metastasis analyzed by whole-genome sequencing (WGS), and 67 trio sets involving brain metastases analyzed with whole-exome sequencing (WES) which were separated into 38 WES-Non-Lung cancers, 6 WES-NSCLC-L (L = low in C>A GOHs) cancers, and 23 WES-NSCLC-H (H = high in C>A GOHs) cancers. In the trio sets of WGS-Liver-M, WES-Non-Lung, WES-NSCLC-L, and WES-NSCLC-H, because nontumor tissues were sampled at > 2 cm from tumor’s edge as controls instead of blood cells, the samples were designated as “N-stage” for comparability with Figs. 1a and 3a. b Patch diagrams tracing SNVs between the N, T, and M samples (see Additional files 9 and 10: Tables S8 and S9 for details of SNVs). M, metastatic tumor. (liver = hepatocellular carcinoma; NSCLC = non-small-cell lung cancer; non-lung = all 12 types of solid tumors in Reference [27] except lung adenocarcinomas and lung squamous carcinomas, see Fig. 1 for abbreviations). c Patch diagrams tracing the SNVs in the fraction of the four WGS sequences in the WGS-Liver-M group that corresponded to all the AluScan-captured regions analyzed in Fig. 1