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Fig. 6 | Human Genomics

Fig. 6

From: Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencing

Fig. 6

The activity of HAS2, PEA3, and MEF2C luciferase reporter genes regulated by TBX2 or TBX3. HEK 293T cells that were co-transfected with vector or wild-type or variant plasmid and a luciferase reporter; transcriptional activity was measured by a luciferase reporter gene detection system. pRL-TK was used as an internal control. a, b Luciferase activity of HAS2, PEA3 promoter regulated by blank vector, and wild-type and variants of TBX2 (n = 3). c Luciferase activity of MEF2C promoter regulated by blank vector, wild-type, and variants of TBX3 (n = 3). #P < 0.01 versus pCDNA or GV141 and *P < 0.05, **P < 0.01 versus WT; data represented here are obtained from three biological replicates

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