Fig. 6From: Identification of TBX2 and TBX3 variants in patients with conotruncal heart defects by target sequencingThe activity of HAS2, PEA3, and MEF2C luciferase reporter genes regulated by TBX2 or TBX3. HEK 293T cells that were co-transfected with vector or wild-type or variant plasmid and a luciferase reporter; transcriptional activity was measured by a luciferase reporter gene detection system. pRL-TK was used as an internal control. a, b Luciferase activity of HAS2, PEA3 promoter regulated by blank vector, and wild-type and variants of TBX2 (n = 3). c Luciferase activity of MEF2C promoter regulated by blank vector, wild-type, and variants of TBX3 (n = 3). #P < 0.01 versus pCDNA or GV141 and *P < 0.05, **P < 0.01 versus WT; data represented here are obtained from three biological replicatesBack to article page