Fig. 5
From: Trans-activation-based risk assessment of BRCA1 BRCT variants with unknown clinical significance
Mean relative expression (RQ) of variant GAL4 DBD:BRCA1(aa 1396–1863) fusion protein in transfected cell lines. a HEK293T and b MDA-MB-231 (green bars) with corresponding TA activities (black dots). RQ is displayed as the relative expression between pcDNA3 GAL4 DBD:BRCA1(aa 1396–1863) and GAPDH. Error bars represent standard deviations (nRQ = 6, nTA ≤ 18). The graph illustrates the independence between RQ and TA activity and is based on three qPCR experiments and three to four TA assay experiments