From: Preimplantation genetic diagnosis and screening (PGD/S) using a semiconductor sequencing platform
Base index | Sample types | Kits (mean ± SD) | Kits (no. of samples) | ||
---|---|---|---|---|---|
SurePlex | DOP-PCR | SurePlex | DOP-PCR | ||
GC content | 0.448 ± 0.004 | 0.414 ± 0.006 | 26 | 66 | |
CV(1 Mb) | 15 pg DNA | 0.133 ± 0.01 | 0.11 ± 0.014 | 12 | 25 |
Single-cell DNA | 0.117 ± 0.012 | 0.094 ± 0.013 | 3 | 4 | |
Duplication ratio | 15 pg DNA | 0.187 ± 0.039 | 0.199 ± 0.059 | 12 | 25 |
Single-cell DNA | 0.185 ± 0.052 | 0.185 ± 0.05 | 3 | 4 | |
Unique mapped reads ratio | 15 pg DNA | 0.517 ± 0.01 | 0.515 ± 0.057 | 12 | 25 |
Single-cell DNA | 0.523 ± 0.061 | 0.479 ± 0.023 | 3 | 4 | |
Unique mapped reads number | 15 pg DNA | 4,137,564 ± 517,233 | 3,953,195 ± 650,395 | 12 | 25 |
Single-cell DNA | 2,883,315 ± 241,218 | 3,574,140 ± 797,115 | 3 | 4 | |
Theory coverage | 15 pg DNA | 0.215 ± 0.025 | 0.194 ± 0.034 | 12 | 25 |
Single-cell DNA | 0.162 ± 0.015 | 0.179 ± 0.036 | 3 | 4 | |
Actual coverage | 15 pg DNA | 0.133 ± 0.012 | 0.102 ± 0.018 | 12 | 25 |
Single-cell DNA | 0.108 ± 0.008 | 0.096 ± 0.017 | 3 | 4 | |
Minus of theory and actual | 15 pg DNA | 0.082 ± 0.02 | 0.091 ± 0.033 | 12 | 25 |
Single-cell DNA | 0.054 ± 0.008 | 0.083 ± 0.025 | 3 | 4 |