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Fig. 4 | Human Genomics

Fig. 4

From: Identification of a novel long non-coding RNA within RUNX1 intron 5

Fig. 4

A novel long non-coding RNA was detected in hematopoietic cell lines. RT-PCR was performed to evaluate the expression of the previously identified long non-coding RNA in four cell lines, two hematopoietic (KG-1 and K562) and two non-hematopoietic (Colo320 and HeLa) (a). RUNX1 gene mRNA expression was evaluated in the same way (b, bottom panel) and also the expression of the reference gene SNRPD3 mRNA (c). A representation of RUNX1 mRNA is included, indicating the position of the primers used, flanking intron 5 (b, top panel). C(+), positive control; O(dT), cDNA synthesized using oligo(dT) as template; -RT. minus RT control; Neg, negative control (water as a template)

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