Fig. 3
From: Genetic variants of VEGFR-1 gene promoter in acute myocardial infarction
Relative transcriptional activity of wild type and variant VEGFR-1 gene promoters. Wild-type and variant VEGFR-1 gene promoters were cloned into reporter gene vector pGL3 and transfected into cultured cells. The transfected cells were collected, and dual-luciferase activities were assayed. Empty vector pGL3-basic was used as a negative control. Transcriptional activity of the wild-type VEGFR-1 gene promoter was designed as 100%. Relative activities of variant VEGFR-1 gene promoters were calculated. a Relative activities of wild type and variant VEGFR-1 gene promoters in HEK-293 cells. b Relative activities of wild-type and variant VEGFR-1 gene promoters in H9c2 cells. Lanes 1, pGL3-basic; 2, pGL3-WT; 3, pGL3-28494900A; 4, pGL3-28495138C; 5, pGL3-28495341G; 6, pGL3-28,495,445 T; 7, pGL3-28,495,796 T; 8, pGL3-28,495,805 T; 9, pGL3-28495356A; and 10, pGL3-28495678A. WT wild type, AMI AMI patients, CTR healthy controls. *P < 0.05, compared to pGL3-WT; **P < 0.01, compared to PGL3-WT