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Table 4 PCR primers for the VEGFR-1 gene promoter and 5′-UTR region

From: Genetic variants of VEGFR-1 gene promoter in acute myocardial infarction

PCR primers Sequences Location Position Products
Sequencing
VEGFR-1-F1 5′-ACATCCCTCTGACGGGTTCCA-3′ 28496221 − 1092 bp 770 bp
VEGFR-1-R1 5′-GTAAGCCGGGTGGAGGGAGT-3′ 28495471 − 338 bp  
VEGFR-1-F2 5′-GCCTCAGTCCTCCGTGCCAAGA-3′ 28495608 − 479 bp 788 bp
VEGFR-1-R2 5′-ATGGTCAGCTACTGGGACACCGG-3′ 28494821 + 308 bp  
Functioning
VEGFR-1-F 5′-(KpnI)-GCTCCGTGCAGCCAGGACGA-3′ 28,496,151 − 1022 bp 1307 bp
VEGFR-1-R 5′-(HindIII)-GTGAGCGCGACGCGGCCT-3′ 28,494,845 + 284 bp  
  1. PCR primers are designed based on the genomic DNA sequence of the VEGFR-1 gene (NC_000013.11). The transcription start is at the position of 28495128 (+ 1). PCR polymerase chain reaction