TY - JOUR AU - Lorenz, David R. AU - Misra, Vikas AU - Gabuzda, Dana PY - 2019 DA - 2019/11/28 TI - Transcriptomic analysis of monocytes from HIV-positive men on antiretroviral therapy reveals effects of tobacco smoking on interferon and stress response systems associated with depressive symptoms JO - Human Genomics SP - 59 VL - 13 IS - 1 AB - Tobacco smoking induces immunomodulatory and pro-inflammatory effects associated with transcriptome changes in monocytes and other immune cell types. While smoking is prevalent in HIV-infected (HIV+) individuals, few studies have investigated its effects on gene expression in this population. Here, we report whole-transcriptome analyses of 125 peripheral blood monocyte samples from ART-treated HIV+ and uninfected (HIV−) men enrolled in the Multicenter AIDS Cohort Study (MACS) (n = 25 HIV+ smokers, n = 60 HIV+ non-smokers, n = 40 HIV− non-smoking controls). Gene expression profiling was performed using Illumina HumanHT-12 Expression BeadChip microarrays. Differential expression analysis was performed with weighted linear regression models using the R limma package, followed by functional enrichment and Ingenuity Pathway analyses. SN - 1479-7364 UR - https://doi.org/10.1186/s40246-019-0247-x DO - 10.1186/s40246-019-0247-x ID - Lorenz2019 ER -