Fig. 2

MiR-204 sequence, processing, and target sites. a Sequence alignment of human (MIR204) and mouse (Mir204) miR-204 genes. The 5p and 3p arms are shaded gray. The 7-nucleotide seed regions are underlined. Nucleotides critical for Drosha cleavage (C32, T92) and Dicer cleavage (T54, G71) are shown in blue and green, respectively. The n.37C > T transition underlying RDICC is shown in red. b Precursor (pre)miR-204 stem formation between the 5p and 3p arms. c Processed miR-204-5p aligned with binding sites in the mRNA 3′-untranslated regions (UTRs) of human TRPM3 and mouse Trpm3. Asterisks (*) denote identical nucleotides. Colons (:) denote base pairing. Dashes (−) denote sequence gaps