An investigation of genetic polymorphisms in heparan sulfate proteoglycan core proteins and key modification enzymes in an Australian Caucasian multiple sclerosis population

Table 2 Assay details and SNP information including RFLP fragment sizes where appropriate

SNP number	Gene	Forward primers	Reverse primers	Chr	Chr position	Amplicon length (bp)	Variation	T_a (°C)	RFLP fragment sizes (bp)	Accession number	Assay type
rs11546829	EXT1	5’ ACAGCCCCTTCCTTACCTGT 3'	5’ GGAAGTAAGGTCAGCCAAACC 3'	8	118847782	397	G/A	51	115, 281	NT_008046.16	RFLP
rs2623047	SULF1	5’ GGGATGCACAGAAACCCTAA 3'	5’ TGTGGCAAACAGTGAAGAGC 3'	8	70378496	291	C/T	57	212, 78	NT_008183.19	RFLP
rs1131351	SDC1	5’ TGCTGTACCGCATGAAGAAG 3'	5’ GCTGTGGTGGAAAGGTCCTA 3'	2	20402380	354	C/G	62	259, 94	NT_015926.15	RFLP
rs7333912	GPC5	5’ GGAAACATAACAAAGTTTGCAATC 3’	5’ TGGGGAGGGATAGGAAGATAAA 3’	13	91874131	120	C/G	49	N/A	NT_009952.14	HRM
rs10492503	GPC5	5' CTTCAATACTCTTGCTTGAATCGT 3'	5' CCGTAATTTGTGAGATATACCTTC 3'	13	92885097	115	A/T	58	N/A	NT_009952.14	HRM
rs9523787	GPC5	5’ TTCCTAGTTGATTGTTGAAGAGA 3’	5’ TGTAACCTTGATTTTCTTTCTAGT 3’	13	93363760	105	G/T	49	N/A	NT_009952.14	HRM
rs17267815	GPC6	5' ATGAGAGGGCTTCCATATAATCAT 3'	5' GGCAACAGTTTTGGAAGAAACA 3'	13	94153058	129	A/G	58	N/A	NT_009952.14	HRM
rs9524260	GPC6	5’ GACAGCCAGTGAATGTAGATAGGA 3’	5’ Biotin-CAAATAACAGGAAGCTCAG 3’	13	94513790	105	G/A	56	N/A	NT_009952.14	Pyro
		Sequencing primer	5' CAAATAACAGGAAGCTCAG 3'

ISSN: 1479-7364