Fig. 2From: Hydroxymethylation and tumors: can 5-hydroxymethylation be used as a marker for tumor diagnosis and treatment?Detecting 5hmC biomarkers in cfDNA of human cancers. The workflow of 5hmC-Seal profiling from cfDNA is shown. Purified cfDNA is ligated with standard sequencing adapters. 5hmC-containing cfDNA fragments are selectively labeled and captured, followed by PCR amplification and next-generation sequencing (NGS)Back to article page