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Fig. 1 | Human Genomics

Fig. 1

From: Mild dyserythropoiesis and β-like globin gene expression imbalance due to the loss of histone chaperone ASF1B

Fig. 1

ASF1B is downregulated in the primary erythroid progenitors carrying the p.K288X KLF1 variant. a Expression analysis from publicly available microarray data (GSE22109) [16] derived from cultured primary erythroid cells (HEPs) of Maltese family members with HPFH (green bars, n = 3) and normal family members (gray bars, n = 3). Family members with HPFH display reduction of BCL11A, ASF1B, E2F2, E2F4, CD44, and HBB and increased HBG/(HBB+HBG) ratio. Mean and standard deviation are depicted. T test p values are indicated. *p < 0.05; **p < 0.005; ***p < 0.0005. b Analysis of KLF1, BCL11A, and ASF1B expression by RT-qPCR on HEPs derived from buffy coats from healthy individuals and transduced with shRNA lentiviruses targeting KLF1 and BCL11A. Knockdown of either factor does not influence significantly the ASF1B expression. Expression levels are normalized setting expression levels of mock knockdown controls at 100. Mean and standard deviation are depicted. Shadowed box at 100 corresponds to the expression levels in mock knockdown controls. T test p values are indicated. *p < 0.05; **p < 0.005; ***p < 0.0005

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