Table 2 List of primers used for real-time PCR to validate microarray analysis
Target gene | Primer sequence | Accession number | Product size |
|---|---|---|---|
GAPDH | F: CTCATTTCCTGGTATGACAACGA R: CTTCCTCTTGTGCTCTTG | NM_001357943.2 | 122 bp |
CTNNB1 | F: AATGCTTGGTTCACCAGTG R: GGCAGTCTGTCGTAATAGCC | NM_001330729.2 | 176 bp |
MAPK3 | F: TGACCATATCTGCTACTTCCTC R: GGTATAGCCCTTGGAGTTCAG | NM_001040056.3 | 250 bp |
ITGAV | F: GCAACAGGCAATAGAGAT R: TGCTGAATCCTCCTTGACAA | NM_002210.5 | 262 bp |
ITGA5 | F: GCTGTGACTACTTTGCCGTG R: CGAGTTGTTGAGATTCTTGCTG | NM_002205.5 | 176 bp |
CCNB1 | F: GCTGGGTGTAGGTCCTTG R: CCTGCCATGTTGATCTTCG | NM_031966.4 | 149 bp |
TLR4 | F: TGATGTCTGCCTCGCGCCTG R: AACCACCTCCACGCAGGGCT | NM_138554.5 | 98 bp |