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Fig. 3 | Human Genomics

Fig. 3

From: C-X-C motif chemokine 16, modulated by microRNA-545, aggravates myocardial damage and affects the inflammatory responses in myocardial infarction

Fig. 3

MiR-545 can directly target CXCL16 and inhibit the expression level of CXCL16 in H/R-stimulated human neonatal cardiomyocytes. a Relative expression of miR-545 in MI (n = 4) and control (n = 3) based on the GSE24548 data set, P < 0.0001. b Expression of miR-545 in vitro H/R cell model, n = 3 independent experiments. **P < 0.01 vs. control group. c Relative luciferase activity was determined using dual-luciferase reporter gene assay, n = 3 independent experiments. **P < 0.01 vs. negative control (NC). d Relative expression of miR-545 in cardiomyocytes transfected with miR-545 inhibitor and mimic, n = 3 independent experiments. **P < 0.01 vs. control group. e–h qRT-PCR and western blot experiments were performed to examine the CXCL16 mRNA and protein expression after diverse transfection, n = 3 independent experiments. **P < 0.01 vs. control and ##P < 0.01 vs. si-CXCL16 or CXCL16. All data were exhibited as mean ± standard deviation (SD)

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Human Genomics

ISSN: 1479-7364