Fig. 3

TUG1 acted as a sponge of miR-137. A The putative binding site between TUG1 and miR137 was predicted by Starbase. B The relative luciferase activity of TUG1 3′-UTR WT/MUT in HEK-293T cells was detected by the dual luciferase reporter assay. C HemECs were transfected with sh-TUG1-1, sh-TUG1-2, or sh-NC. MiR-137 expression was assessed by qRT-PCR. D, E HemECs were transfected with miR-137 mimics/inhibitor, and corresponding negative controls. The expression of miR-137 (D) and TUG1 (E) was evaluated by qRT-PCR. F MiR-137 expression in infant hemangioma tissues (n = 30) and adjacent normal subcutaneous tissues (n = 30) was assessed by qRT-PCR. G The correlation between the level of TUG1 and miR-137 in infant hemangioma tissues (n = 30) was evaluated by Spearman’s correlation analysis. Data were derived from three independent experiments and expressed as mean ± SD. * P < 0.01