Fig. 4

MiR-137 inhibited HemEC proliferation, migration, and invasion via regulating IGFBP5. A–D HemECs were transfected with miR-137 mimics and NC mimics. Cell proliferation was evaluated by CCK-8 assay (A) and colony formation assay (B). C Cell migration was evaluated by the wound healing assay. D Cell invasion was evaluated by Transwell assay. E The interactional region between miR-137 and IGFBP5 was predicted by Starbase. F The relative luciferase activity of IGFBP5 3′-UTR WT/MUT in HEK-293T cells was detected by the dual luciferase reporter assay. G HemECs were transfected with TUG1 (the overexpressing vector for TUG1) or empty vector pcDNA3.1. TUG1 expression was evaluated by qRT-PCR. H–J HemECs were transfected with miR-137 mimics, NC mimics, or co-transfected with miR-137 mimics and the empty vector, or miR137 mimics and TUG1 (the overexpressing vector for TUG1). IGFBP5 expression was evaluated by qRT-PCR (H) and Western blot (I). J IGFBP5 expression was evaluated by immunofluorescence using an anti-IGFBP5 antibody. Magnification, × 200; scale bar = 100 μm. Data were derived from three independent experiments and expressed as mean ± SD. * P < 0.05, ** P < 0.01