Fig. 6
From: LncRNA-TUG1 promotes the progression of infantile hemangioma by regulating miR-137/IGFBP5 axis
TUG1 knockdown inhibited HemEC proliferation, migration, and invasion via regulating IGFBP5. A, B HemECs were transfected with IGFBP5 (the overexpressing vector for IGFBP5) or the empty vector pcDNA3.1. The transfection efficiency was confirmed by qRT-PCR (A) and Western blot (B). C–H HemECs were transfected with sh-TUG1-1, sh-NC, or co-transfected with sh-TUG1-1 and the empty vector pcDNA3.1, or sh-TUG1-1 and IGFBP5 (the overexpressing vector for IGFBP5). IGFBP5 expression was evaluated by qRT-PCR (C) and Western blot (D). Cell proliferation was evaluated by CCK-8 assay (E) and colony formation assay (F). G Cell migration was evaluated by the wound healing assay. H Cell invasion was evaluated by Transwell assay. Data were derived from three independent experiments and expressed as mean ± SD. * P < 0.05, ** P < 0.01