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Fig. 2 | Human Genomics

Fig. 2

From: RNA-seq driven expression and enrichment analysis to investigate CVD genes with associated phenotypes among high-risk heart failure patients

Fig. 2

RNA-seq data processing pipeline. Used FastQC for quality checking; Trimmomatic to remove adapters and low-quality sequences; SAMtools to sort and index sequences; MarkDuplicates to remove duplicates; CollectInsertSizeMetrics to compute size distribution and read orientation of paired-end libraries; HISAT with Bowtie2 to align sequences to the human reference genome; and RSEM to quantify and identify differentially expressed genes by aligning reads to reference de novo transcriptome assemblies

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Human Genomics

ISSN: 1479-7364