Fig. 3
From: Impaired phosphate transport in SLC34A2 variants in patients with pulmonary alveolar microlithiasis
Phosphate transport activity in Xenopus laevis oocytes 3 days after injection of cRNA encoding wild-type (WT) or mutated c-Myc-tagged hNaPi-IIb constructs. NI is non-injected oocytes serving as additional controls. Data presented are values from 3 to 4 independent experiments with 7–10 oocytes per group incubated for 10 min in ND96 containing 1 mM cold Pi and 32P. Vertical bars represent the median transport activity. There was a statistically significant between-group difference (p = 0.0001, Kruskal–Wallis test by ranks). Post-hoc analysis revealed a statistically significant difference between WT and both mutants and NI control oocytes (p < 0.05, Dunn's multiple comparison test). 32Pi uptake levels in oocytes expressing mutants were similar to non-injected oocytes (p > 0.05, Dunn's multiple comparison test)