Fig. 1
From: Identification of the ataxin-1 interaction network and its impact on spinocerebellar ataxia type 1
Validation of proteins associated with ataxin-1. A–F Co-immunoprecipitation experiments were performed with HEK-293T cells expressing wild-type ataxin-1. In each case, Flag was immunoprecipitated, and then Myc and Flag were immunoblotted. Co-immunoprecipitation-western assays showed that wild-type ataxin-1 interacts with GNAS (B), MCM2 (C), and TMEM206 (D), but could not interact with UTP18 (A), FASN (E), and MED23 (F). G–L Co-immunoprecipitation experiments were performed with HEK-293T cells expressing mutant ataxin-1. Similarly, Flag was immunoprecipitated, and then Myc and Flag were immunoblotted in each case. Co-immunoprecipitation-western assays demonstrated that mutant ataxin-1 could not interact with SND1 (G), RAB11A (H), SPCS2 (I), ACAT1 (J), HP1BP3 (K), and FASN (L). Molecular weight (kDa): Myc-UTP18 (62), Myc-GNAS (46), Myc-MCM2 (104), Myc-TMEM206 (40), Myc-FASN (273), Myc-MED23 (130), Myc-SND1 (104), Myc-RAB11A (25), Myc-SPCS2 (25), Myc-ACAT1 (46), Myc-HP1BP3 (63), Myc-FASN (273), Flag-ataxin-1-W (94), Flag-ataxin-1-M (120)